Peripheral blood expansion of early progenitor cells after high-dose cyclophosphamide and rhGM-CSF

Corrado Tarella, Dario Ferrero, Marco Bregni, Salvatore Siena, Eugenio Gallo, Alessandro Pileri, Alessandro M. Gianni

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In 20 patients with non-Hodgkin lymphoma or breast cancer, high-dose cyclophosphamide induced, during the post-nadir period of rapid leucocyte recovery, on median day 19 about a 30-fold increase in the peak concentration of granulocyte-macrophage (CFU-GM) and erythroid (BFU-E) colony-forming cells, and an even higher increase in the more immature pluripotent progenitors (CFU-Mix, 72-fold). After infusion of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF), peak concentration was reached earlier (median day 15) and with further enhancements (159, 116 and 283-fold, respectively, in the number of CFU-GM, BFU-E and CFU-Mix). Most CFU-GM were immature, lacking the differentiation antigen CD15, and gave rise to large myeloid colonies, reflecting a high proliferative capacity of the founder cells. Very immature maphosphamide-resistant progenitors were detectable. The marked expansion in the circulating pool was predictable and reliable, allowing harvesting, after two or three leukaphereses, of sufficient haematopoietic progenitors for autologous bone-marrow reconstitution.

Original languageEnglish
Pages (from-to)22-27
Number of pages6
JournalEuropean Journal of Cancer and Clinical Oncology
Issue number1
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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