PDGFRA immunostaining can help in the diagnosis of gastrointestinal stromal tumors

Francesca Miselli; Clara Millefanti; Elena Conca; Tiziana Negri; Cristina Piacenza; Marco A. Pierotti; Elena Tamborini; Silvana Pilotti

doi:10.1097/PAS.0b013e31815c47e8

PDGFRA immunostaining can help in the diagnosis of gastrointestinal stromal tumors

Francesca Miselli, Clara Millefanti, Elena Conca, Tiziana Negri, Cristina Piacenza, Marco A. Pierotti, Elena Tamborini, Silvana Pilotti

Fondazione IRCCS Istituto Nazionale dei Tumori

Research output: Contribution to journal › Article › peer-review

Abstract

Gastrointestinal stromal tumors (GISTs) are characterized by the presence of activating mutations affecting the c-Kit or the PDGFRA gene. Although these mutations are mutually exclusive, their proteins are coexpressed in many GISTs with various modulations of immunostaining depending on which gene is mutated. CD117 expression is currently considered a sensitive (although not entirely specific) marker of KIT activation, but there is no consensus concerning the reliability of PDGFRA antibody. Our database contains 236 molecularly analyzed GISTs, and we here describe the 180 cases that underwent KIT/PDGFRA immunophenotyping. By correlating the immunophenotype with the molecular status of the genes expected to be involved, we observed the coexpression of KIT and PDGFRA in the majority of the mutated c-Kit and wild-type c-Kit/PDGFRA GISTs, whereas the -/+ immunophenotype (0% vs. 48.6%) and PDGFRA dotlike immunostaining (P

Original language	English
Pages (from-to)	738-743
Number of pages	6
Journal	American Journal of Surgical Pathology
Volume	32
Issue number	5
DOIs	https://doi.org/10.1097/PAS.0b013e31815c47e8
Publication status	Published - May 2008

Keywords

Dotlike decoration
GIST
Immunohistochemistry
Molecular analyses
PDGFRA

ASJC Scopus subject areas

Anatomy
Pathology and Forensic Medicine

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10.1097/PAS.0b013e31815c47e8

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title = "PDGFRA immunostaining can help in the diagnosis of gastrointestinal stromal tumors",

abstract = "Gastrointestinal stromal tumors (GISTs) are characterized by the presence of activating mutations affecting the c-Kit or the PDGFRA gene. Although these mutations are mutually exclusive, their proteins are coexpressed in many GISTs with various modulations of immunostaining depending on which gene is mutated. CD117 expression is currently considered a sensitive (although not entirely specific) marker of KIT activation, but there is no consensus concerning the reliability of PDGFRA antibody. Our database contains 236 molecularly analyzed GISTs, and we here describe the 180 cases that underwent KIT/PDGFRA immunophenotyping. By correlating the immunophenotype with the molecular status of the genes expected to be involved, we observed the coexpression of KIT and PDGFRA in the majority of the mutated c-Kit and wild-type c-Kit/PDGFRA GISTs, whereas the -/+ immunophenotype (0% vs. 48.6%) and PDGFRA dotlike immunostaining (P",

keywords = "Dotlike decoration, GIST, Immunohistochemistry, Molecular analyses, PDGFRA",

author = "Francesca Miselli and Clara Millefanti and Elena Conca and Tiziana Negri and Cristina Piacenza and Pierotti, {Marco A.} and Elena Tamborini and Silvana Pilotti",

year = "2008",

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AU - Miselli, Francesca

AU - Millefanti, Clara

AU - Conca, Elena

AU - Negri, Tiziana

AU - Piacenza, Cristina

AU - Pierotti, Marco A.

AU - Tamborini, Elena

AU - Pilotti, Silvana

PY - 2008/5

Y1 - 2008/5

N2 - Gastrointestinal stromal tumors (GISTs) are characterized by the presence of activating mutations affecting the c-Kit or the PDGFRA gene. Although these mutations are mutually exclusive, their proteins are coexpressed in many GISTs with various modulations of immunostaining depending on which gene is mutated. CD117 expression is currently considered a sensitive (although not entirely specific) marker of KIT activation, but there is no consensus concerning the reliability of PDGFRA antibody. Our database contains 236 molecularly analyzed GISTs, and we here describe the 180 cases that underwent KIT/PDGFRA immunophenotyping. By correlating the immunophenotype with the molecular status of the genes expected to be involved, we observed the coexpression of KIT and PDGFRA in the majority of the mutated c-Kit and wild-type c-Kit/PDGFRA GISTs, whereas the -/+ immunophenotype (0% vs. 48.6%) and PDGFRA dotlike immunostaining (P

AB - Gastrointestinal stromal tumors (GISTs) are characterized by the presence of activating mutations affecting the c-Kit or the PDGFRA gene. Although these mutations are mutually exclusive, their proteins are coexpressed in many GISTs with various modulations of immunostaining depending on which gene is mutated. CD117 expression is currently considered a sensitive (although not entirely specific) marker of KIT activation, but there is no consensus concerning the reliability of PDGFRA antibody. Our database contains 236 molecularly analyzed GISTs, and we here describe the 180 cases that underwent KIT/PDGFRA immunophenotyping. By correlating the immunophenotype with the molecular status of the genes expected to be involved, we observed the coexpression of KIT and PDGFRA in the majority of the mutated c-Kit and wild-type c-Kit/PDGFRA GISTs, whereas the -/+ immunophenotype (0% vs. 48.6%) and PDGFRA dotlike immunostaining (P

KW - Dotlike decoration

KW - GIST

KW - Immunohistochemistry

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PDGFRA immunostaining can help in the diagnosis of gastrointestinal stromal tumors

Abstract

Keywords

ASJC Scopus subject areas

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