Overexpression of the human EGF receptor confers an EGF-dependent transformed phenotype to NIH 3T3 cells

Pier Paolo Di Fiore, Jacalyn H. Pierce, Timothy P. Fleming, Rachel Hazan, Axel Ullrich, C. Richter King, Joseph Schlessinger, Stuart A. Aaronson

Research output: Contribution to journalArticlepeer-review


The epidermal growth factor receptor (EGFR) gene is frequently amplified and/or overexpressed in human malignancies. To investigate the biological effects of its overexpression, we constructed a eukaryotic vector containing human EGFR cDNA. Introduction of this construct led to reconstitution of functional EGF receptors in NR6 mutant cells, which are normally devoid of this receptor. Transfection of NIH 3T3 resulted in no significant alterations in growth properties. However, EGF addition led to the formation of densely growing transformed foci in liquid culture and colonies in semisolid medium. NIH 3T3-EGFR clonal lines, which expressed the EGF at 500- to 1000-fold levels over control NIH 3T3 cells, demonstrated a marked increase in DNA synthesis in response to EGF. Thus EGF receptor overexpression appears to amplify normal EGF signal transduction. Finally, high levels of EGFR expression, which conferred a transformed phenotype to NIH 3T3 cells in the presence of ligand, were demonstrated in representative human tumor cell lines that contained amplified copies of the EGFR gene.

Original languageEnglish
Pages (from-to)1063-1070
Number of pages8
Issue number6
Publication statusPublished - Dec 24 1987

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology


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