Abstract
The authors have evaluated the use of anti-human immunoglobulins conjugated with peroxidase, alkaline-phosphatase, glucose-oxidase as a diagnostic tool for essaying autoantibodies. A comparison study was done using the classical indirect immunofluorescence technique. Immunoenzymatic techniques, of minor cost compared to immunofluorescence, have revealed a better appreciation of the global and particular structure of the tissue in examination. From the three enzymes examined, peroxidase revealed itself as first choice, because, other than having on overlapping sensitivity to immunofluorescence, it can allow a further ultrastructural study.
| Translated title of the contribution | Observations about the use of different types of enzymes in immunohistological diagnosis of autoimmune diseases. Comparative study with indirect immunofluorescence technique |
|---|---|
| Original language | Italian |
| Title of host publication | Annali Sclavo - Rivista di Microbiologia e di Immunologia |
| Pages | 561-567 |
| Number of pages | 7 |
| Volume | 21 |
| Edition | 4 |
| Publication status | Published - 1979 |
ASJC Scopus subject areas
- Medicine(all)