Nuclear lamin A in rotator cuff tear margin tenocytes: an antiapoptotic and cell mechanostat factor

Stefano Gumina, Barbara Peruzzi, Martina Leopizzi, Natale Porta, Valeria Di Maio, Carlo Della Rocca, Vittorio Candela

Research output: Contribution to journalArticlepeer-review


BACKGROUND: The network of intermediate filament proteins underlying the inner nuclear membrane forms the nuclear lamin. A- and B-type lamins are the major components of the nuclear lamina. Lamins function in many nuclear activities. The role of lamin A and transcription factors (NF-kB) as anti-apoptotic is well documented. Recently, lamin A has also been considered as a mechanosensor protein that is able to maintain nuclear integrity from mechanical insults. We aimed to verify how lamin A expression varies in healthy cuff cells and in those with different-sized tears where various mechanical stresses are present.

METHODS: Forty-three patients with rotator cuff tear (RCT) [23M-20F, mean age (SD): 63.5 (6.1)] were enrolled. Tissue samples excised from the most medial point of tear margins were analyzed for lamin A expression by immunohistochemistry. Controls were represented by samples obtained by normal supraspinatus tendons excised from patients submitted to reverse shoulder prosthesis implant [8M-7F, mean age (SD): 67.9 (7.1)]. The intensity of staining was graded, and an H-score was assigned. Statistical analysis was performed.

RESULTS: Our study revealed a moderate intensity of lamin A in the healthy cuff tendons, a higher expression of this protein in the small tears, and a significant decrease of lamin A with increasing tear size (p < 0.0001).

CONCLUSIONS: Our study emphasizes the importance of early repair of small RCTs since nuclear stability is maintained, and the cellular function is protected by lamin A overexpression. High re-tear of massive cuff repair could be due to cellular apoptosis and nuclear modifications induced by lamin A lack.


Original languageEnglish
Pages (from-to)413
JournalJournal of Orthopaedic Surgery and Research
Issue number1
Publication statusPublished - Jun 30 2021


  • Aged
  • Apoptosis
  • Case-Control Studies
  • Female
  • Humans
  • Immunohistochemistry
  • Lamin Type A/metabolism
  • Male
  • Middle Aged
  • Rotator Cuff/cytology
  • Rotator Cuff Injuries/metabolism
  • Tenocytes/metabolism


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