Non-invasive tissue diagnostics using a multimodal spectroscopic device based on fiber probe

Riccardo Cicchi, Suresh Anand, Susanna Rossari, Alessandro Sturiale, Flavio Giordano, Vincenzo De Giorgi, Vincenza Maio, Daniela Massi, Gabriella Nesi, Anna Maria Buccoliero, Francesco Tonelli, Renzo Guerrini, Nicola Pimpinelli, Francesco Saverio Pavone

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Two different optical fiber probes for combined Raman and fluorescence spectroscopic measurements were designed, developed and used for tissue diagnostics. Two visible laser diodes were used for fluorescence spectroscopy, whereas a laser diode emitting in the NIR was used for Raman spectroscopy. The two probes were based on fiber bundles with a central multimode optical fiber, used for delivering light to the tissue, and 24 surrounding optical fibers for signal collection. Both fluorescence and Raman spectra were acquired using the same detection unit, based on a cooled CCD camera, connected to a spectrograph. The two probes were successfully employed for diagnosing melanocytic lesions in a good agreement with common routine histology. The obtained results demonstrated that the multimodal approach is crucial for improving diagnostic capabilities. Further investigations were performed on colon and brain tissue samples in order to have a benchmark for diagnosing a broader range of tissue lesions and malignancies. The system presented here can improve diagnostic capabilities on a broad range of tissues and has the potential of being used for endoscopic inspections in the near future.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
ISBN (Print)9781628410778
Publication statusPublished - 2014
EventBiophotonics: Photonic Solutions for Better Health Care IV - Brussels, Belgium
Duration: Apr 14 2014Apr 17 2014


OtherBiophotonics: Photonic Solutions for Better Health Care IV


  • (170.6935)Tissue characterization
  • (300.6280) Spectroscopy
  • (300.6450) Spectroscopy
  • fluorescence and luminescence
  • Raman

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics


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