Neural stem cell transplantation induces stroke recovery by upregulating glutamate transporter GLT-1 in astrocytes

Marco Bacigaluppi, Gianluca Luigi Russo, Luca Peruzzotti-Jametti, Silvia Rossi, Stefano Sandrone, Erica Butti, Roberta de Ceglia, Andrea Bergamaschi, Caterina Motta, Mattia Gallizioli, Valeria Studer, Emanuela Colombo, Cinthia Farina, Giancarlo Comi, Letterio Salvatore Politi, Luca Muzio, Claudia Villani, Roberto William Invernizzi, Dirk M. Hermann, Diego CentonzeGianvito Martino

Research output: Contribution to journalArticlepeer-review


Ischemic stroke is the leading cause of disability, but effective therapies are currently widely lacking. Rescovery from stroke is very much dependent on the possibility to develop treatments able to both halt the neurodegenerative process as well as to foster adaptive tissue plasticity. Here we show that ischemic mice treated with neural precursor cell (NPC) transplantation had on neurophysiological analysis, early after treatment, reduced presynaptic release of glutamate within the ipsilesional corticospinal tract (CST), and an enhanced NMDA-mediated excitatory transmission in the contralesional CST. Concurrently, NPC-treated mice displayed a reduced CST degeneration, increased axonal rewiring, and augmented dendritic arborization, resulting in long-term functional amelioration persisting up to 60 d after ischemia. The enhanced functional and structural plasticity relied on the capacity of transplanted NPCs to localize in the peri-ischemic and ischemic area, to promote the upregulation of the glial glutamate transporter 1 (GLT-1) on astrocytes and to reduce peri-ischemic extracellular glutamate. The upregulation of GLT-1 induced by transplanted NPCs was found to rely on the secretion of VEGF by NPCs. Blocking VEGF during the first week after stroke reduced GLT-1 upregulation as well as long-term behavioral recovery in NPC-treated mice. Our results show that NPC transplantation, by modulating the excitatory–inhibitory balance and stroke microenvi-ronment, is a promising therapy to ameliorate disability, to promote tissue recovery and plasticity processes after stroke.

Original languageEnglish
Pages (from-to)10529-10544
Number of pages16
JournalJournal of Neuroscience
Issue number41
Publication statusPublished - Oct 12 2016


  • Ischemia
  • Neurophysiology
  • Plasticity
  • Recovery
  • Stem cell
  • Transplantation

ASJC Scopus subject areas

  • Neuroscience(all)


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