Nerve growth factor modulates in vitro the expression and release of TGF-β1 by amniotic membrane

Marco Coassin; Alessandro Lambiase; Alessandra Micera; Paola Tirassa; Luigi Aloe; Stefano Bonini

doi:10.1007/s00417-005-0098-0

Nerve growth factor modulates in vitro the expression and release of TGF-β1 by amniotic membrane

Marco Coassin, Alessandro Lambiase, Alessandra Micera, Paola Tirassa, Luigi Aloe, Stefano Bonini

Fondazione G.B. Bietti

Research output: Contribution to journal › Article › peer-review

Abstract

Purpose: To determinate the basal production of NGF and TGF-β1 by amniotic membrane (AM) and to verify the presence of NGF receptors (trkA ^NGFR and p75 ^NTR) in AM. Thereafter, to evaluate in an in vitro model if increasing concentrations of NGF are able to stimulate AM to produce and release TGF-β1. Methods: Immunohistochemistry, ELISA, in situ hybridization and PCR analysis for NGF, TGF-β1, trkA ^NGFR and p75 ^NTR were performed to study their presence in AM. Amniotic membranes were stimulated in vitro with increasing concentrations of NGF. After 24 h, the amount of TGF- β1 in the AM tissue and in the culture medium was investigate. Results: AM expressed TGF-β1, NGF, trkA ^NGFR and p75 ^NTR mRNAs and proteins and released basal amounts of NGF and TGF-β1 in the medium. Stimulation of AM by addition of NGF induced a significant (P

Original language	English
Pages (from-to)	485-491
Number of pages	7
Journal	Graefe's Archive for Clinical and Experimental Ophthalmology
Volume	244
Issue number	4
DOIs	https://doi.org/10.1007/s00417-005-0098-0
Publication status	Published - Apr 2006

Keywords

Amniotic membrane
Amniotic membrane transplantation
Nerve growth factor
Transforming growth factor-β1

ASJC Scopus subject areas

Ophthalmology

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10.1007/s00417-005-0098-0

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title = "Nerve growth factor modulates in vitro the expression and release of TGF-β1 by amniotic membrane",

abstract = "Purpose: To determinate the basal production of NGF and TGF-β1 by amniotic membrane (AM) and to verify the presence of NGF receptors (trkA NGFR and p75 NTR) in AM. Thereafter, to evaluate in an in vitro model if increasing concentrations of NGF are able to stimulate AM to produce and release TGF-β1. Methods: Immunohistochemistry, ELISA, in situ hybridization and PCR analysis for NGF, TGF-β1, trkA NGFR and p75 NTR were performed to study their presence in AM. Amniotic membranes were stimulated in vitro with increasing concentrations of NGF. After 24 h, the amount of TGF- β1 in the AM tissue and in the culture medium was investigate. Results: AM expressed TGF-β1, NGF, trkA NGFR and p75 NTR mRNAs and proteins and released basal amounts of NGF and TGF-β1 in the medium. Stimulation of AM by addition of NGF induced a significant (P",

keywords = "Amniotic membrane, Amniotic membrane transplantation, Nerve growth factor, Transforming growth factor-β1",

author = "Marco Coassin and Alessandro Lambiase and Alessandra Micera and Paola Tirassa and Luigi Aloe and Stefano Bonini",

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T1 - Nerve growth factor modulates in vitro the expression and release of TGF-β1 by amniotic membrane

AU - Coassin, Marco

AU - Lambiase, Alessandro

AU - Micera, Alessandra

AU - Tirassa, Paola

AU - Aloe, Luigi

AU - Bonini, Stefano

PY - 2006/4

Y1 - 2006/4

N2 - Purpose: To determinate the basal production of NGF and TGF-β1 by amniotic membrane (AM) and to verify the presence of NGF receptors (trkA NGFR and p75 NTR) in AM. Thereafter, to evaluate in an in vitro model if increasing concentrations of NGF are able to stimulate AM to produce and release TGF-β1. Methods: Immunohistochemistry, ELISA, in situ hybridization and PCR analysis for NGF, TGF-β1, trkA NGFR and p75 NTR were performed to study their presence in AM. Amniotic membranes were stimulated in vitro with increasing concentrations of NGF. After 24 h, the amount of TGF- β1 in the AM tissue and in the culture medium was investigate. Results: AM expressed TGF-β1, NGF, trkA NGFR and p75 NTR mRNAs and proteins and released basal amounts of NGF and TGF-β1 in the medium. Stimulation of AM by addition of NGF induced a significant (P

AB - Purpose: To determinate the basal production of NGF and TGF-β1 by amniotic membrane (AM) and to verify the presence of NGF receptors (trkA NGFR and p75 NTR) in AM. Thereafter, to evaluate in an in vitro model if increasing concentrations of NGF are able to stimulate AM to produce and release TGF-β1. Methods: Immunohistochemistry, ELISA, in situ hybridization and PCR analysis for NGF, TGF-β1, trkA NGFR and p75 NTR were performed to study their presence in AM. Amniotic membranes were stimulated in vitro with increasing concentrations of NGF. After 24 h, the amount of TGF- β1 in the AM tissue and in the culture medium was investigate. Results: AM expressed TGF-β1, NGF, trkA NGFR and p75 NTR mRNAs and proteins and released basal amounts of NGF and TGF-β1 in the medium. Stimulation of AM by addition of NGF induced a significant (P

KW - Amniotic membrane

KW - Amniotic membrane transplantation

KW - Nerve growth factor

KW - Transforming growth factor-β1

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Nerve growth factor modulates in vitro the expression and release of TGF-β1 by amniotic membrane

Abstract

Keywords

ASJC Scopus subject areas

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