Myogenic regional responsiveness to cholinergic and vipergic stimulation in human colon

M. A. Maselli, P. Trisolini, C. Petitta, D. Lorusso, A. Cicenia, A. Scirocco, F. Pezzolla, C. Severi

Research output: Contribution to journalArticlepeer-review


Background Differences in the actions of enteric neurotransmitters on colonic circular and longitudinal muscle layers have not been clearly determined, nor the possible existence of intrinsic myogenic phenotypes that might contribute to regional differences in human colon motor activity. The aim of this study was to analyze the direct pharmaco-mechanical coupling of carbachol (CCh) and vasoactive intestinal polypeptide (VIP) on human colonic smooth muscle strips and cells. Methods Circular and longitudinal muscle strips and cells were obtained from 15 human specimens of ascending and sigmoid colon. Both isometric tension on muscle strips and contraction and relaxation on cells were measured in response to increasing CCh and VIP concentrations. Key Results Circular muscle strips of ascending colon were more sensitive to the effect of CCh than that of sigmoid colon, EC50 values being, respectively, 4.15μmolL-1 and 8.47μmolL-1 (P50: 0.041 and 0.15μmolL-1, respectively, P50: 0.043 and 0.09μmolL-1, respectively, P50: 44.85 and 75.24nmolL-1, respectively, P

Original languageEnglish
JournalNeurogastroenterology and Motility
Issue number9
Publication statusPublished - Sept 2012


  • Ascending and sigmoid colon
  • Carbachol
  • Human
  • Smooth muscle
  • Vasoactive intestinal polypeptide

ASJC Scopus subject areas

  • Endocrine and Autonomic Systems
  • Gastroenterology
  • Physiology


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