TY - JOUR
T1 - Myogenic regional responsiveness to cholinergic and vipergic stimulation in human colon
AU - Maselli, M. A.
AU - Trisolini, P.
AU - Petitta, C.
AU - Lorusso, D.
AU - Cicenia, A.
AU - Scirocco, A.
AU - Pezzolla, F.
AU - Severi, C.
PY - 2012/9
Y1 - 2012/9
N2 - Background Differences in the actions of enteric neurotransmitters on colonic circular and longitudinal muscle layers have not been clearly determined, nor the possible existence of intrinsic myogenic phenotypes that might contribute to regional differences in human colon motor activity. The aim of this study was to analyze the direct pharmaco-mechanical coupling of carbachol (CCh) and vasoactive intestinal polypeptide (VIP) on human colonic smooth muscle strips and cells. Methods Circular and longitudinal muscle strips and cells were obtained from 15 human specimens of ascending and sigmoid colon. Both isometric tension on muscle strips and contraction and relaxation on cells were measured in response to increasing CCh and VIP concentrations. Key Results Circular muscle strips of ascending colon were more sensitive to the effect of CCh than that of sigmoid colon, EC50 values being, respectively, 4.15μmolL-1 and 8.47μmolL-1 (P50: 0.041 and 0.15μmolL-1, respectively, P50: 0.043 and 0.09μmolL-1, respectively, P50: 44.85 and 75.24nmolL-1, respectively, P
AB - Background Differences in the actions of enteric neurotransmitters on colonic circular and longitudinal muscle layers have not been clearly determined, nor the possible existence of intrinsic myogenic phenotypes that might contribute to regional differences in human colon motor activity. The aim of this study was to analyze the direct pharmaco-mechanical coupling of carbachol (CCh) and vasoactive intestinal polypeptide (VIP) on human colonic smooth muscle strips and cells. Methods Circular and longitudinal muscle strips and cells were obtained from 15 human specimens of ascending and sigmoid colon. Both isometric tension on muscle strips and contraction and relaxation on cells were measured in response to increasing CCh and VIP concentrations. Key Results Circular muscle strips of ascending colon were more sensitive to the effect of CCh than that of sigmoid colon, EC50 values being, respectively, 4.15μmolL-1 and 8.47μmolL-1 (P50: 0.041 and 0.15μmolL-1, respectively, P50: 0.043 and 0.09μmolL-1, respectively, P50: 44.85 and 75.24nmolL-1, respectively, P
KW - Ascending and sigmoid colon
KW - Carbachol
KW - Human
KW - Smooth muscle
KW - Vasoactive intestinal polypeptide
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U2 - 10.1111/j.1365-2982.2012.01927.x
DO - 10.1111/j.1365-2982.2012.01927.x
M3 - Article
C2 - 22672206
AN - SCOPUS:84865404945
SN - 1350-1925
VL - 24
JO - Neurogastroenterology and Motility
JF - Neurogastroenterology and Motility
IS - 9
ER -