TY - JOUR
T1 - Multimodal imaging for a theranostic approach in a murine model of B-cell lymphoma with engineered nanoparticles
AU - Torino, Enza
AU - Auletta, Luigi
AU - Vecchione, Donatella
AU - Orlandella, Francesca Maria
AU - Salvatore, Giuliana
AU - Iaccino, Enrico
AU - Fiorenza, Dario
AU - Grimaldi, Anna Maria
AU - Sandomenico, Annamaria
AU - Albanese, Sandra
AU - Sarnataro, Daniela
AU - Gramanzini, Matteo
AU - Palmieri, Camillo
AU - Scala, Giuseppe
AU - Quinto, Ileana
AU - Netti, Paolo Antonio
AU - Salvatore, Marco
AU - Greco, Adelaide
PY - 2017/11/22
Y1 - 2017/11/22
N2 - Nanoparticles (NPs) are a promising tool for in vivo multimodality imaging and theranostic applications. Hyaluronic acid (HA)-based NPs have numerous active groups that make them ideal as tumor-targeted carriers. The B-lymphoma neoplastic cells express on their surfaces a clone-specific immunoglobulin receptor (Ig-BCR). The peptide A20-36 (pA20-36) selectively binds to the Ig-BCR of A20 lymphoma cells. In this work, we demonstrated the ability of core-shell chitosan-HA-NPs decorated with pA20-36 to specifically target A20 cells and reduce the tumor burden in a murine xenograft model. We monitored tumor growth using high-frequency ultrasonography and demonstrated targeting specificity and kinetics of the NPs via in vivo fluorescent reflectance imaging. This result was also confirmed by ex vivo magnetic resonance imaging and confocal microscopy. In conclusion, we demonstrated the ability of NPs loaded with fluorescent and paramagnetic tracers to act as multimodal imaging contrast agents and hence as a non-toxic, highly specific theranostic system.
AB - Nanoparticles (NPs) are a promising tool for in vivo multimodality imaging and theranostic applications. Hyaluronic acid (HA)-based NPs have numerous active groups that make them ideal as tumor-targeted carriers. The B-lymphoma neoplastic cells express on their surfaces a clone-specific immunoglobulin receptor (Ig-BCR). The peptide A20-36 (pA20-36) selectively binds to the Ig-BCR of A20 lymphoma cells. In this work, we demonstrated the ability of core-shell chitosan-HA-NPs decorated with pA20-36 to specifically target A20 cells and reduce the tumor burden in a murine xenograft model. We monitored tumor growth using high-frequency ultrasonography and demonstrated targeting specificity and kinetics of the NPs via in vivo fluorescent reflectance imaging. This result was also confirmed by ex vivo magnetic resonance imaging and confocal microscopy. In conclusion, we demonstrated the ability of NPs loaded with fluorescent and paramagnetic tracers to act as multimodal imaging contrast agents and hence as a non-toxic, highly specific theranostic system.
KW - Engineered nanoparticles
KW - Fluorescence imaging
KW - Magnetic resonance
KW - Mouse model
KW - Preclinical imaging
KW - Theranostic
UR - http://www.scopus.com/inward/record.url?scp=85039975318&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85039975318&partnerID=8YFLogxK
U2 - 10.1016/j.nano.2017.11.016
DO - 10.1016/j.nano.2017.11.016
M3 - Article
AN - SCOPUS:85039975318
SN - 1549-9634
VL - 14
SP - 483
EP - 491
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
IS - 2
ER -