Multimodal imaging for a theranostic approach in a murine model of B-cell lymphoma with engineered nanoparticles

Enza Torino, Luigi Auletta, Donatella Vecchione, Francesca Maria Orlandella, Giuliana Salvatore, Enrico Iaccino, Dario Fiorenza, Anna Maria Grimaldi, Annamaria Sandomenico, Sandra Albanese, Daniela Sarnataro, Matteo Gramanzini, Camillo Palmieri, Giuseppe Scala, Ileana Quinto, Paolo Antonio Netti, Marco Salvatore, Adelaide Greco

Research output: Contribution to journalArticlepeer-review


Nanoparticles (NPs) are a promising tool for in vivo multimodality imaging and theranostic applications. Hyaluronic acid (HA)-based NPs have numerous active groups that make them ideal as tumor-targeted carriers. The B-lymphoma neoplastic cells express on their surfaces a clone-specific immunoglobulin receptor (Ig-BCR). The peptide A20-36 (pA20-36) selectively binds to the Ig-BCR of A20 lymphoma cells. In this work, we demonstrated the ability of core-shell chitosan-HA-NPs decorated with pA20-36 to specifically target A20 cells and reduce the tumor burden in a murine xenograft model. We monitored tumor growth using high-frequency ultrasonography and demonstrated targeting specificity and kinetics of the NPs via in vivo fluorescent reflectance imaging. This result was also confirmed by ex vivo magnetic resonance imaging and confocal microscopy. In conclusion, we demonstrated the ability of NPs loaded with fluorescent and paramagnetic tracers to act as multimodal imaging contrast agents and hence as a non-toxic, highly specific theranostic system.

Original languageEnglish
Pages (from-to)483-491
Number of pages9
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Issue number2
Publication statusE-pub ahead of print - Nov 22 2017


  • Engineered nanoparticles
  • Fluorescence imaging
  • Magnetic resonance
  • Mouse model
  • Preclinical imaging
  • Theranostic

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science


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