Molecular immune signatures of HIV-1 vaccines in human PBMCs

Alessandro Monaco; Francesco M. Marincola; Marianna Sabatino; Zoltan Pos; Maria Lina Tornesello; David F. Stroncek; Ena Wang; George K. Lewis; Franco M. Buonaguro; Luigi Buonaguro

doi:10.1016/j.febslet.2009.07.060

Molecular immune signatures of HIV-1 vaccines in human PBMCs

Alessandro Monaco, Francesco M. Marincola, Marianna Sabatino, Zoltan Pos, Maria Lina Tornesello, David F. Stroncek, Ena Wang, George K. Lewis, Franco M. Buonaguro, Luigi Buonaguro

Istituto Nazionale Tumori Fondazione G. Pascale

Research output: Contribution to journal › Article › peer-review

Abstract

The global transcriptional profile of peripheral blood mononuclear cells (PBMCs) stimulated with HIV candidate vaccine (virus-like particles, VLPs) has been evaluated in HIV-infected patients with low/high viral load compared to healthy volunteers. Baseline activation of chemokine production was observed in PBMC from HIV-infected patients and innate immune stimulation with HIV-VLPs was not blunted. The immune profile among HIV-infected patients was found to be qualitatively similar but quantitatively extremely variable. This diversity was independent of viral load and it might be dependent on individual immunogenetic traits or concurrent immunological status. This ex vivo screening strategy represents an efficient tool for guiding modifications/optimizations of vaccination strategies and understanding failures in individuals enrolled in clinical trials.

Original language	English
Pages (from-to)	3004-3008
Number of pages	5
Journal	FEBS Letters
Volume	583
Issue number	18
DOIs	https://doi.org/10.1016/j.febslet.2009.07.060
Publication status	Published - Sept 17 2009

Keywords

HIV-1
Immunogenomics
Peripheral blood mononuclear cell
Vaccine

ASJC Scopus subject areas

Biochemistry
Biophysics
Cell Biology
Genetics
Molecular Biology
Structural Biology

Access to Document

10.1016/j.febslet.2009.07.060

Cite this

@article{33bb88dc77234e38993d698ff9a73520,

title = "Molecular immune signatures of HIV-1 vaccines in human PBMCs",

abstract = "The global transcriptional profile of peripheral blood mononuclear cells (PBMCs) stimulated with HIV candidate vaccine (virus-like particles, VLPs) has been evaluated in HIV-infected patients with low/high viral load compared to healthy volunteers. Baseline activation of chemokine production was observed in PBMC from HIV-infected patients and innate immune stimulation with HIV-VLPs was not blunted. The immune profile among HIV-infected patients was found to be qualitatively similar but quantitatively extremely variable. This diversity was independent of viral load and it might be dependent on individual immunogenetic traits or concurrent immunological status. This ex vivo screening strategy represents an efficient tool for guiding modifications/optimizations of vaccination strategies and understanding failures in individuals enrolled in clinical trials.",

keywords = "HIV-1, Immunogenomics, Peripheral blood mononuclear cell, Vaccine",

author = "Alessandro Monaco and Marincola, {Francesco M.} and Marianna Sabatino and Zoltan Pos and Tornesello, {Maria Lina} and Stroncek, {David F.} and Ena Wang and Lewis, {George K.} and Buonaguro, {Franco M.} and Luigi Buonaguro",

year = "2009",

month = sep,

day = "17",

doi = "10.1016/j.febslet.2009.07.060",

language = "English",

volume = "583",

pages = "3004--3008",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "18",

}

TY - JOUR

T1 - Molecular immune signatures of HIV-1 vaccines in human PBMCs

AU - Monaco, Alessandro

AU - Marincola, Francesco M.

AU - Sabatino, Marianna

AU - Pos, Zoltan

AU - Tornesello, Maria Lina

AU - Stroncek, David F.

AU - Wang, Ena

AU - Lewis, George K.

AU - Buonaguro, Franco M.

AU - Buonaguro, Luigi

PY - 2009/9/17

Y1 - 2009/9/17

N2 - The global transcriptional profile of peripheral blood mononuclear cells (PBMCs) stimulated with HIV candidate vaccine (virus-like particles, VLPs) has been evaluated in HIV-infected patients with low/high viral load compared to healthy volunteers. Baseline activation of chemokine production was observed in PBMC from HIV-infected patients and innate immune stimulation with HIV-VLPs was not blunted. The immune profile among HIV-infected patients was found to be qualitatively similar but quantitatively extremely variable. This diversity was independent of viral load and it might be dependent on individual immunogenetic traits or concurrent immunological status. This ex vivo screening strategy represents an efficient tool for guiding modifications/optimizations of vaccination strategies and understanding failures in individuals enrolled in clinical trials.

AB - The global transcriptional profile of peripheral blood mononuclear cells (PBMCs) stimulated with HIV candidate vaccine (virus-like particles, VLPs) has been evaluated in HIV-infected patients with low/high viral load compared to healthy volunteers. Baseline activation of chemokine production was observed in PBMC from HIV-infected patients and innate immune stimulation with HIV-VLPs was not blunted. The immune profile among HIV-infected patients was found to be qualitatively similar but quantitatively extremely variable. This diversity was independent of viral load and it might be dependent on individual immunogenetic traits or concurrent immunological status. This ex vivo screening strategy represents an efficient tool for guiding modifications/optimizations of vaccination strategies and understanding failures in individuals enrolled in clinical trials.

KW - HIV-1

KW - Immunogenomics

KW - Peripheral blood mononuclear cell

KW - Vaccine

UR - http://www.scopus.com/inward/record.url?scp=69949114850&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69949114850&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2009.07.060

DO - 10.1016/j.febslet.2009.07.060

M3 - Article

C2 - 19665024

AN - SCOPUS:69949114850

SN - 0014-5793

VL - 583

SP - 3004

EP - 3008

JO - FEBS Letters

JF - FEBS Letters

IS - 18

ER -

Molecular immune signatures of HIV-1 vaccines in human PBMCs

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this