Molecular analysis of the Imatinib-induced complete cytogenetic response in chronic myelogenous leukemia

Maurizio Miglino; Raffaella Grasso; Riccardo Varaldo; Giuseppina Fugazza; Nicoletta Colombo; Marino Clavio; Letizia Canepa; Anna Garuti; Adalberto Ibatici; Ivana Pierri; Filippo Ballerini; Mario Sessarego; Marco Gobbi

doi:10.1080/10428190500525607

Molecular analysis of the Imatinib-induced complete cytogenetic response in chronic myelogenous leukemia

Maurizio Miglino, Raffaella Grasso, Riccardo Varaldo, Giuseppina Fugazza, Nicoletta Colombo, Marino Clavio, Letizia Canepa, Anna Garuti, Adalberto Ibatici, Ivana Pierri, Filippo Ballerini, Mario Sessarego, Marco Gobbi

A.O.U. San Martino - IST, Istituto Nazionale Ricerca sul Cancro (GENOVA)

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this study was to elucidate the relationship between clonal and normal haematopoiesis in chronic myelogenous leukemia (CML). Thirteen female patients who reached complete cytogenetic response (CCR) after Imatinib® treatment were studied. Although all the study patients exhibited a polyclonal pattern of X inactivation, p210 BCR/ABL transcript remained detectable in all cases by nested RT-PCR. This study also demonstrated the presence of p190 transcript in nine out of 13 study patients. A longer follow-up analysis is needed to clarify the prognostic value of these results. The recent observation that clonal cytogenetic abnormalities may occur in CML patients in polyclonal remission after imatinib suggests that a neoplastic stem cell lacking BCR/ABL rearrangement may acquire further cytogenetic alterations other than Philadelphia chromosome.

Original language	English
Pages (from-to)	1348-1351
Number of pages	4
Journal	Leukemia and Lymphoma
Volume	47
Issue number	7
DOIs	https://doi.org/10.1080/10428190500525607
Publication status	Published - Jul 2006

Keywords

BCR/ABL
Chronic myelogenous leukemia
Clonality
Humara
Imatinib

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

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10.1080/10428190500525607

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title = "Molecular analysis of the Imatinib-induced complete cytogenetic response in chronic myelogenous leukemia",

abstract = "The aim of this study was to elucidate the relationship between clonal and normal haematopoiesis in chronic myelogenous leukemia (CML). Thirteen female patients who reached complete cytogenetic response (CCR) after Imatinib{\textregistered} treatment were studied. Although all the study patients exhibited a polyclonal pattern of X inactivation, p210 BCR/ABL transcript remained detectable in all cases by nested RT-PCR. This study also demonstrated the presence of p190 transcript in nine out of 13 study patients. A longer follow-up analysis is needed to clarify the prognostic value of these results. The recent observation that clonal cytogenetic abnormalities may occur in CML patients in polyclonal remission after imatinib suggests that a neoplastic stem cell lacking BCR/ABL rearrangement may acquire further cytogenetic alterations other than Philadelphia chromosome.",

keywords = "BCR/ABL, Chronic myelogenous leukemia, Clonality, Humara, Imatinib",

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AU - Miglino, Maurizio

AU - Grasso, Raffaella

AU - Varaldo, Riccardo

AU - Fugazza, Giuseppina

AU - Colombo, Nicoletta

AU - Clavio, Marino

AU - Canepa, Letizia

AU - Garuti, Anna

AU - Ibatici, Adalberto

AU - Pierri, Ivana

AU - Ballerini, Filippo

AU - Sessarego, Mario

AU - Gobbi, Marco

PY - 2006/7

Y1 - 2006/7

N2 - The aim of this study was to elucidate the relationship between clonal and normal haematopoiesis in chronic myelogenous leukemia (CML). Thirteen female patients who reached complete cytogenetic response (CCR) after Imatinib® treatment were studied. Although all the study patients exhibited a polyclonal pattern of X inactivation, p210 BCR/ABL transcript remained detectable in all cases by nested RT-PCR. This study also demonstrated the presence of p190 transcript in nine out of 13 study patients. A longer follow-up analysis is needed to clarify the prognostic value of these results. The recent observation that clonal cytogenetic abnormalities may occur in CML patients in polyclonal remission after imatinib suggests that a neoplastic stem cell lacking BCR/ABL rearrangement may acquire further cytogenetic alterations other than Philadelphia chromosome.

AB - The aim of this study was to elucidate the relationship between clonal and normal haematopoiesis in chronic myelogenous leukemia (CML). Thirteen female patients who reached complete cytogenetic response (CCR) after Imatinib® treatment were studied. Although all the study patients exhibited a polyclonal pattern of X inactivation, p210 BCR/ABL transcript remained detectable in all cases by nested RT-PCR. This study also demonstrated the presence of p190 transcript in nine out of 13 study patients. A longer follow-up analysis is needed to clarify the prognostic value of these results. The recent observation that clonal cytogenetic abnormalities may occur in CML patients in polyclonal remission after imatinib suggests that a neoplastic stem cell lacking BCR/ABL rearrangement may acquire further cytogenetic alterations other than Philadelphia chromosome.

KW - BCR/ABL

KW - Chronic myelogenous leukemia

KW - Clonality

KW - Humara

KW - Imatinib

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Molecular analysis of the Imatinib-induced complete cytogenetic response in chronic myelogenous leukemia

Abstract

Keywords

ASJC Scopus subject areas

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