Model of interaction of the IL-1 receptor accessory protein IL-1RAcP with the IL-1β/IL-1RI complex

Rita Casadio; Elisabetta Frigimelica; Paola Bossù; Detlef Neumann; Michael U. Martin; Aldo Tagliabue; Diana Boraschi

doi:10.1016/S0014-5793(01)02515-7

Model of interaction of the IL-1 receptor accessory protein IL-1RAcP with the IL-1β/IL-1RI complex

Rita Casadio, Elisabetta Frigimelica, Paola Bossù, Detlef Neumann, Michael U. Martin, Aldo Tagliabue, Diana Boraschi

Fondazione Santa Lucia

Research output: Contribution to journal › Article › peer-review

Abstract

A preliminary model has been calculated for the activating interaction of the interleukin 1 receptor (IL-1R) accessory protein IL-1RAcP with the ligand/receptor complex IL-1β/IL-1R_I. First, IL-1RAcP was modeled on the crystal structure of IL-1R_I bound to IL-1β. Then, the IL-1RAcP model was docked using specific programs to the crystal structure of the IL-1β/IL-1R_I complex. Two types of models were predicted, with comparable probability. Experimental data obtained with the use of IL-1β peptides and antibodies, and with mutated IL-1β proteins, support the BACK model, in which IL-1RAcP establishes contacts with the back of IL-1R_I wrapped around IL-1β.

Original language	English
Pages (from-to)	65-68
Number of pages	4
Journal	FEBS Letters
Volume	499
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-5793(01)02515-7
Publication status	Published - Jun 15 2001

Keywords

Accessory protein
Interleukin 1
Molecular modeling
Receptor chain

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/S0014-5793(01)02515-7

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@article{6b2d444d41154b7fa41bd02c47a75966,

title = "Model of interaction of the IL-1 receptor accessory protein IL-1RAcP with the IL-1β/IL-1RI complex",

abstract = "A preliminary model has been calculated for the activating interaction of the interleukin 1 receptor (IL-1R) accessory protein IL-1RAcP with the ligand/receptor complex IL-1β/IL-1RI. First, IL-1RAcP was modeled on the crystal structure of IL-1RI bound to IL-1β. Then, the IL-1RAcP model was docked using specific programs to the crystal structure of the IL-1β/IL-1RI complex. Two types of models were predicted, with comparable probability. Experimental data obtained with the use of IL-1β peptides and antibodies, and with mutated IL-1β proteins, support the BACK model, in which IL-1RAcP establishes contacts with the back of IL-1RI wrapped around IL-1β.",

keywords = "Accessory protein, Interleukin 1, Molecular modeling, Receptor chain",

author = "Rita Casadio and Elisabetta Frigimelica and Paola Boss{\`u} and Detlef Neumann and Martin, {Michael U.} and Aldo Tagliabue and Diana Boraschi",

year = "2001",

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doi = "10.1016/S0014-5793(01)02515-7",

language = "English",

volume = "499",

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T1 - Model of interaction of the IL-1 receptor accessory protein IL-1RAcP with the IL-1β/IL-1RI complex

AU - Casadio, Rita

AU - Frigimelica, Elisabetta

AU - Bossù, Paola

AU - Neumann, Detlef

AU - Martin, Michael U.

AU - Tagliabue, Aldo

AU - Boraschi, Diana

PY - 2001/6/15

Y1 - 2001/6/15

N2 - A preliminary model has been calculated for the activating interaction of the interleukin 1 receptor (IL-1R) accessory protein IL-1RAcP with the ligand/receptor complex IL-1β/IL-1RI. First, IL-1RAcP was modeled on the crystal structure of IL-1RI bound to IL-1β. Then, the IL-1RAcP model was docked using specific programs to the crystal structure of the IL-1β/IL-1RI complex. Two types of models were predicted, with comparable probability. Experimental data obtained with the use of IL-1β peptides and antibodies, and with mutated IL-1β proteins, support the BACK model, in which IL-1RAcP establishes contacts with the back of IL-1RI wrapped around IL-1β.

AB - A preliminary model has been calculated for the activating interaction of the interleukin 1 receptor (IL-1R) accessory protein IL-1RAcP with the ligand/receptor complex IL-1β/IL-1RI. First, IL-1RAcP was modeled on the crystal structure of IL-1RI bound to IL-1β. Then, the IL-1RAcP model was docked using specific programs to the crystal structure of the IL-1β/IL-1RI complex. Two types of models were predicted, with comparable probability. Experimental data obtained with the use of IL-1β peptides and antibodies, and with mutated IL-1β proteins, support the BACK model, in which IL-1RAcP establishes contacts with the back of IL-1RI wrapped around IL-1β.

KW - Accessory protein

KW - Interleukin 1

KW - Molecular modeling

KW - Receptor chain

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U2 - 10.1016/S0014-5793(01)02515-7

DO - 10.1016/S0014-5793(01)02515-7

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AN - SCOPUS:0035876314

SN - 0014-5793

VL - 499

SP - 65

EP - 68

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Model of interaction of the IL-1 receptor accessory protein IL-1RAcP with the IL-1β/IL-1RI complex

Abstract

Keywords

ASJC Scopus subject areas

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