TY - JOUR
T1 - Melatonin and sirtuins in buccal epithelium
T2 - Potential biomarkers of aging and age-related pathologies
AU - Carbone, Annalucia
AU - Linkova, Natalia
AU - Polyakova, Victoria
AU - Mironova, Ekaterina
AU - Hashimova, Ulduz
AU - Gadzhiev, Ahmed
AU - Safikhanova, Khatira
AU - Kvetnaia, Tatiana
AU - Krylova, Julia
AU - Tarquini, Roberto
AU - Mazzoccoli, Gianluigi
AU - Kvetnoy, Igor
N1 - Funding Information:
Funding: This study was supported by the “5 × 1000” voluntary contribution and by a grant from the Italian Ministry of Health (Ricerca Corrente 2018, 2019, 2020) to G.M. and by RFFI grant 18-54-06012 Az_a; SDF (Azerbaijan) grants: EIF-BGM-4-RFTF-1/2017-21/19/3, and EIF-KETPL-2-2015-1(25)-56/36/3-M-54 to I.K.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/11/1
Y1 - 2020/11/1
N2 - Melatonin (MT) and sirtuins (SIRT) are geroprotective molecules that hold back the aging process and the development of age-related diseases, including cardiovascular pathologies. Buccal epithelium (BE) sampling is a non-invasive procedure, yielding highly informative material for evaluating the expression of genes and proteins as well as the synthesis of molecules. Among these, MT and SIRTs are valuable markers of the aging process and age-related pathologies. The purpose of this study was to examine age-related expression patterns of these signaling molecules, in particular MT, SIRT1, SIRT3, and SIRT6 in BE of subjects of different ages with and without arterial hypertension (AH). We used real-time polymerase chain reaction (RT-PCR) and immunofluorescence analysis by confocal microscopy. We found that MT immunofluorescence intensity in BE decreases with aging, more evidently in AH patients. SIRT3 and SIRT6 genes expression and immunofluorescence intensity in BE was decreased in aging controls. In AH patients, SIRT1, SIRT3, and SIRT6 gene expression and immunofluorescence intensity in BE was decreased in relation to age and in comparison with age-matched controls. In conclusion, the evaluation of MT and sirtuins in BE could provide a non-invasive method for appraising the aging process, also when accompanied by AH.
AB - Melatonin (MT) and sirtuins (SIRT) are geroprotective molecules that hold back the aging process and the development of age-related diseases, including cardiovascular pathologies. Buccal epithelium (BE) sampling is a non-invasive procedure, yielding highly informative material for evaluating the expression of genes and proteins as well as the synthesis of molecules. Among these, MT and SIRTs are valuable markers of the aging process and age-related pathologies. The purpose of this study was to examine age-related expression patterns of these signaling molecules, in particular MT, SIRT1, SIRT3, and SIRT6 in BE of subjects of different ages with and without arterial hypertension (AH). We used real-time polymerase chain reaction (RT-PCR) and immunofluorescence analysis by confocal microscopy. We found that MT immunofluorescence intensity in BE decreases with aging, more evidently in AH patients. SIRT3 and SIRT6 genes expression and immunofluorescence intensity in BE was decreased in aging controls. In AH patients, SIRT1, SIRT3, and SIRT6 gene expression and immunofluorescence intensity in BE was decreased in relation to age and in comparison with age-matched controls. In conclusion, the evaluation of MT and sirtuins in BE could provide a non-invasive method for appraising the aging process, also when accompanied by AH.
KW - Aging
KW - Arterial hypertension
KW - Buccal epithelium
KW - Melatonin
KW - Sirtuins
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U2 - 10.3390/ijms21218134
DO - 10.3390/ijms21218134
M3 - Article
C2 - 33143333
AN - SCOPUS:85095572887
SN - 1661-6596
VL - 21
SP - 1
EP - 12
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 21
M1 - 8134
ER -