TY - JOUR
T1 - Manipulation of radiation-induced bystander effect in prostate adenocarcinoma by dose and tumor differentiation grade
T2 - In vitro study
AU - Tubin, Slavisa
AU - Valeriani, Maurizio
AU - Salerno, Gerardo
AU - Bracci, Stefano
AU - Stoppacciaro, Antonella
AU - Cardelli, Patrizia
AU - Osti, Mattia Falchetto
AU - De Sanctis, Vitaliana
AU - Minniti, Giuseppe
AU - Enrici, Riccardo Maurizi
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Purpose: This in vitro study evaluated the ability of prostate adenocarcinoma (ADC) cells to induce radiation-induced bystander effect (RIBE) exploring the factors that may be responsible and affect its intensity. The idea was to mimic a strong, clinically applicable RIBE that could lead to the development of innovative approaches in modern radiotherapy of prostate cancer, especially for those patients with hormone-refractory ADC in which radiotherapy might have a limited role. Materials and methods: Two human prostate cancer cell lines of different differentiation, PC-3 and DU-145, have been irradiated using wide range of doses to obtain radiation-conditioned medium (RCM), which was used to treat the unirradiated cells and to evaluate the cytokines level. Using a trypan blue dye exclusion method, cell growth was assessed. Results: Prostate ADC cells were able to induce RIBE; intensity depended on dose and cell differentiation. RIBE intensity of DU-145 was not correlated with the cytokines level, while for PC-3 Interleukin-6 (IL-6) correlates with strongest RIBE induced by 20 Gy. Conclusions: RIBE can be manipulated by modifying radiation dose and depends on cell differentiation status. IL-6 correlates with RIBE after exposure of PC-3 to a very high dose of radiation, thus indicates its possible involvement in bystander signaling.
AB - Purpose: This in vitro study evaluated the ability of prostate adenocarcinoma (ADC) cells to induce radiation-induced bystander effect (RIBE) exploring the factors that may be responsible and affect its intensity. The idea was to mimic a strong, clinically applicable RIBE that could lead to the development of innovative approaches in modern radiotherapy of prostate cancer, especially for those patients with hormone-refractory ADC in which radiotherapy might have a limited role. Materials and methods: Two human prostate cancer cell lines of different differentiation, PC-3 and DU-145, have been irradiated using wide range of doses to obtain radiation-conditioned medium (RCM), which was used to treat the unirradiated cells and to evaluate the cytokines level. Using a trypan blue dye exclusion method, cell growth was assessed. Results: Prostate ADC cells were able to induce RIBE; intensity depended on dose and cell differentiation. RIBE intensity of DU-145 was not correlated with the cytokines level, while for PC-3 Interleukin-6 (IL-6) correlates with strongest RIBE induced by 20 Gy. Conclusions: RIBE can be manipulated by modifying radiation dose and depends on cell differentiation status. IL-6 correlates with RIBE after exposure of PC-3 to a very high dose of radiation, thus indicates its possible involvement in bystander signaling.
KW - Adenocarcinoma
KW - Bystander effect
KW - Prostate
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U2 - 10.3109/09553002.2015.959667
DO - 10.3109/09553002.2015.959667
M3 - Article
C2 - 25174735
AN - SCOPUS:84922838002
SN - 0955-3002
VL - 91
SP - 166
EP - 171
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
IS - 2
ER -