Long non-coding RNA Linc-RAM enhances myogenic differentiation by interacting with MyoD

Xiaohua Yu; Yong Zhang; Tingting Li; Zhao Ma; Haixue Jia; Qian Chen; Yixia Zhao; Lili Zhai; Ran Zhong; Changyin Li; Xiaoting Zou; Jiao Meng; Antony K. Chen; Pier Lorenzo Puri; Meihong Chen; Dahai Zhu

doi:10.1038/ncomms14016

Long non-coding RNA Linc-RAM enhances myogenic differentiation by interacting with MyoD

Xiaohua Yu, Yong Zhang, Tingting Li, Zhao Ma, Haixue Jia, Qian Chen, Yixia Zhao, Lili Zhai, Ran Zhong, Changyin Li, Xiaoting Zou, Jiao Meng, Antony K. Chen, Pier Lorenzo Puri, Meihong Chen, Dahai Zhu

Fondazione Santa Lucia

Research output: Contribution to journal › Article › peer-review

Abstract

Long non-coding RNAs (lncRNAs) are important regulators of diverse biological processes. Here we report on functional identification and characterization of a novel long intergenic non-coding RNA with MyoD-regulated and skeletal muscle-restricted expression that promotes the activation of the myogenic program, and is therefore termed Linc-RAM (Linc-RNA Activator of Myogenesis). Linc-RAM is transcribed from an intergenic region of myogenic cells and its expression is upregulated during myogenesis. Notably, in vivo functional studies show that Linc-RAM knockout mice display impaired muscle regeneration due to the differentiation defect of satellite cells. Mechanistically, Linc-RAM regulates expression of myogenic genes by directly binding MyoD, which in turn promotes the assembly of the MyoD-Baf60c-Brg1 complex on the regulatory elements of target genes. Collectively, our findings reveal the functional role and molecular mechanism of a lineage-specific Linc-RAM as a regulatory lncRNA required for tissues-specific chromatin remodelling and gene expression.

Original language	English
Article number	14016
Journal	Nature Communications
Volume	8
DOIs	https://doi.org/10.1038/ncomms14016
Publication status	Published - Jan 16 2017

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

Access to Document

10.1038/ncomms14016

Cite this

@article{8d3fe0c1efc24c66afc08942074ceb3a,

title = "Long non-coding RNA Linc-RAM enhances myogenic differentiation by interacting with MyoD",

abstract = "Long non-coding RNAs (lncRNAs) are important regulators of diverse biological processes. Here we report on functional identification and characterization of a novel long intergenic non-coding RNA with MyoD-regulated and skeletal muscle-restricted expression that promotes the activation of the myogenic program, and is therefore termed Linc-RAM (Linc-RNA Activator of Myogenesis). Linc-RAM is transcribed from an intergenic region of myogenic cells and its expression is upregulated during myogenesis. Notably, in vivo functional studies show that Linc-RAM knockout mice display impaired muscle regeneration due to the differentiation defect of satellite cells. Mechanistically, Linc-RAM regulates expression of myogenic genes by directly binding MyoD, which in turn promotes the assembly of the MyoD-Baf60c-Brg1 complex on the regulatory elements of target genes. Collectively, our findings reveal the functional role and molecular mechanism of a lineage-specific Linc-RAM as a regulatory lncRNA required for tissues-specific chromatin remodelling and gene expression.",

author = "Xiaohua Yu and Yong Zhang and Tingting Li and Zhao Ma and Haixue Jia and Qian Chen and Yixia Zhao and Lili Zhai and Ran Zhong and Changyin Li and Xiaoting Zou and Jiao Meng and Chen, {Antony K.} and Puri, {Pier Lorenzo} and Meihong Chen and Dahai Zhu",

year = "2017",

month = jan,

day = "16",

doi = "10.1038/ncomms14016",

language = "English",

volume = "8",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "NLM (Medline)",

}

TY - JOUR

T1 - Long non-coding RNA Linc-RAM enhances myogenic differentiation by interacting with MyoD

AU - Yu, Xiaohua

AU - Zhang, Yong

AU - Li, Tingting

AU - Ma, Zhao

AU - Jia, Haixue

AU - Chen, Qian

AU - Zhao, Yixia

AU - Zhai, Lili

AU - Zhong, Ran

AU - Li, Changyin

AU - Zou, Xiaoting

AU - Meng, Jiao

AU - Chen, Antony K.

AU - Puri, Pier Lorenzo

AU - Chen, Meihong

AU - Zhu, Dahai

PY - 2017/1/16

Y1 - 2017/1/16

N2 - Long non-coding RNAs (lncRNAs) are important regulators of diverse biological processes. Here we report on functional identification and characterization of a novel long intergenic non-coding RNA with MyoD-regulated and skeletal muscle-restricted expression that promotes the activation of the myogenic program, and is therefore termed Linc-RAM (Linc-RNA Activator of Myogenesis). Linc-RAM is transcribed from an intergenic region of myogenic cells and its expression is upregulated during myogenesis. Notably, in vivo functional studies show that Linc-RAM knockout mice display impaired muscle regeneration due to the differentiation defect of satellite cells. Mechanistically, Linc-RAM regulates expression of myogenic genes by directly binding MyoD, which in turn promotes the assembly of the MyoD-Baf60c-Brg1 complex on the regulatory elements of target genes. Collectively, our findings reveal the functional role and molecular mechanism of a lineage-specific Linc-RAM as a regulatory lncRNA required for tissues-specific chromatin remodelling and gene expression.

AB - Long non-coding RNAs (lncRNAs) are important regulators of diverse biological processes. Here we report on functional identification and characterization of a novel long intergenic non-coding RNA with MyoD-regulated and skeletal muscle-restricted expression that promotes the activation of the myogenic program, and is therefore termed Linc-RAM (Linc-RNA Activator of Myogenesis). Linc-RAM is transcribed from an intergenic region of myogenic cells and its expression is upregulated during myogenesis. Notably, in vivo functional studies show that Linc-RAM knockout mice display impaired muscle regeneration due to the differentiation defect of satellite cells. Mechanistically, Linc-RAM regulates expression of myogenic genes by directly binding MyoD, which in turn promotes the assembly of the MyoD-Baf60c-Brg1 complex on the regulatory elements of target genes. Collectively, our findings reveal the functional role and molecular mechanism of a lineage-specific Linc-RAM as a regulatory lncRNA required for tissues-specific chromatin remodelling and gene expression.

UR - http://www.scopus.com/inward/record.url?scp=85009458792&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85009458792&partnerID=8YFLogxK

U2 - 10.1038/ncomms14016

DO - 10.1038/ncomms14016

M3 - Article

AN - SCOPUS:85009458792

SN - 2041-1723

VL - 8

JO - Nature Communications

JF - Nature Communications

M1 - 14016

ER -

Long non-coding RNA Linc-RAM enhances myogenic differentiation by interacting with MyoD

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this