Lipid interaction of Tetanus neurotoxin A calorimetric and fluorescence spectroscopy study

Emanuela Calappi; Massimo Masserini; Giampietro Schiavo; Cesare Montecucco; Guido Tettamanti

doi:10.1016/0014-5793(92)81074-V

Lipid interaction of Tetanus neurotoxin A calorimetric and fluorescence spectroscopy study

Emanuela Calappi, Massimo Masserini, Giampietro Schiavo, Cesare Montecucco, Guido Tettamanti

IRCCS Policlinico San Donato

Research output: Contribution to journal › Article › peer-review

Abstract

The interaction of Tetunus toxin with phospholipid vesicles containing gangliosides (GD1a, GD1b or GT1b) or phosphatidic acid has been investigated at neutral or acidic pH. Change in the thermotropic properties of the vesicles occurred only after addition of the toxin at acidic pH, and led to surface binding or membrane insertion of the protein, dependent on the physical state of the membrane. Most remarkably, toxin addition at acidic pH to dipalmitoyl-phosphatidylcholine vesicles containing GT1b ganglioside, caused formation of ganglioside microdomains on the vesicle surface.

Original language	English
Pages (from-to)	107-110
Number of pages	4
Journal	FEBS Letters
Volume	309
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(92)81074-V
Publication status	Published - Sept 7 1992

Keywords

Ganglioside microdomain
Tetanus toxin

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology
Cell Biology
Genetics
Structural Biology

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10.1016/0014-5793(92)81074-V

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abstract = "The interaction of Tetunus toxin with phospholipid vesicles containing gangliosides (GD1a, GD1b or GT1b) or phosphatidic acid has been investigated at neutral or acidic pH. Change in the thermotropic properties of the vesicles occurred only after addition of the toxin at acidic pH, and led to surface binding or membrane insertion of the protein, dependent on the physical state of the membrane. Most remarkably, toxin addition at acidic pH to dipalmitoyl-phosphatidylcholine vesicles containing GT1b ganglioside, caused formation of ganglioside microdomains on the vesicle surface.",

keywords = "Ganglioside microdomain, Tetanus toxin",

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AU - Calappi, Emanuela

AU - Masserini, Massimo

AU - Schiavo, Giampietro

AU - Montecucco, Cesare

AU - Tettamanti, Guido

PY - 1992/9/7

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AB - The interaction of Tetunus toxin with phospholipid vesicles containing gangliosides (GD1a, GD1b or GT1b) or phosphatidic acid has been investigated at neutral or acidic pH. Change in the thermotropic properties of the vesicles occurred only after addition of the toxin at acidic pH, and led to surface binding or membrane insertion of the protein, dependent on the physical state of the membrane. Most remarkably, toxin addition at acidic pH to dipalmitoyl-phosphatidylcholine vesicles containing GT1b ganglioside, caused formation of ganglioside microdomains on the vesicle surface.

KW - Ganglioside microdomain

KW - Tetanus toxin

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Lipid interaction of Tetanus neurotoxin A calorimetric and fluorescence spectroscopy study

Abstract

Keywords

ASJC Scopus subject areas

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