LINC00152 expression in normal and Chronic Lymphocytic Leukemia B cells

S. Matis, M. Rossi, L. Brondolo, M. Cardillo, D. Reverberi, R. Massara, M. Colombo, A. Ibatici, E. Angelucci, T. Vaisitti, S. Bruno, S. Fabris, A. Neri, M. Gentile, F. Morabito, G. Cutrona, P. Briata, R. Gherzi, F. Fais

Research output: Contribution to journalArticlepeer-review


Long non-coding RNAs are emerging as essential regulators of gene expression, but their role in normal and neoplastic B cells is still largely uncharacterized. Here, we report on the expression pattern of the LINC00152 in normal B cells and Chronic Lymphocytic Leukemia B cell clones. Higher LINC00152 levels were consistently observed in memory B cell populations when compared to naïve B cells in the normal tissues analyzed [peripheral blood (PB), tonsils, and spleen]. In addition, independent stimulation via Immunoglobulins (IG), CD40, or Toll-like Receptor 9 (TLR9) upregulated LINC00152 in PB B cells. The expression of LINC00152 in a cohort of 107 early stage Binet A CLL patients was highly variable and did not correlate with known prognostic markers or clinical evolution. TLR9 stimulation, but not CD40 or IG challenge, was able to upregulate LINC00152 expression in CLL cells. In addition, LINC00152 silencing in CLL cell lines expressing LINC00152 failed to induce significant cell survival or apoptosis changes. These data suggest that, in normal B cells, the expression of LINC00152 is regulated by immunomodulatory signals, which are only partially effective in CLL cells. However, LINC00152 does not appear to contribute to CLL cell expansion and/or survival in a cohort of newly diagnosed CLL patients.

Original languageEnglish
Pages (from-to)40-47
Number of pages8
JournalHematological Oncology
Issue number1
Publication statusPublished - 2022


  • Biomarkers, Tumor
  • Humans
  • Leukemia, Lymphocytic, Chronic, B-Cell
  • Palatine Tonsil
  • Prognosis
  • Prospective Studies
  • RNA, Long Noncoding
  • Spleen
  • Survival Rate
  • CD40 antigen
  • long untranslated RNA
  • long untranslated RNA linc00152
  • small interfering RNA
  • toll like receptor 9
  • unclassified drug
  • long non-coding RNA Linc00152, human
  • tumor marker
  • adult
  • animal cell
  • apoptosis
  • Article
  • B lymphocyte subpopulation
  • cancer prognosis
  • cancer survival
  • cell clone
  • cell survival
  • chronic lymphatic leukemia
  • clinical article
  • controlled study
  • early cancer
  • Epstein Barr virus infection
  • fluorescence activated cell sorting
  • follow up
  • genetic transfection
  • germinal center
  • human
  • human cell
  • memory B lymphocyte
  • mRNA expression level
  • nonhuman
  • prospective study
  • real time polymerase chain reaction
  • spleen cell
  • tonsil cell
  • upregulation
  • genetics
  • metabolism
  • palatine tonsil
  • pathology
  • prognosis
  • spleen
  • survival rate


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