TY - JOUR
T1 - Lessons from in vitro perifusion of pancreatic islets isolated from 80 human pancreases
AU - Bertuzzi, F.
AU - Garancini, P.
AU - Socci, C.
AU - Nano, R.
AU - Taglietti, M. V.
AU - Santopinto, M.
AU - Di Carlo, V.
AU - Davalli, A. M.
PY - 1999/11
Y1 - 1999/11
N2 - We report the average insulin response to acute glucose measured by in vitro perifusion of pancreatic islets isolated from 80 consecutive human organs. Different perifusion parameters were considered [basal release, stimulation index (SI), time to peak, incremental area under the curve Δ- AUCa)], and the correlation among them was determined. SI positively correlated with A-AUCa (p <0.001, r = 0.80) while negatively with time to peak (p <0.05, r = -0.23). We also evaluated several variables of the isolation procedure that might affect responsiveness to glucose by human islets. Sex and age of pancreas donors, cold ischemia time, duration of the digestion, collagenase concentration, and lot characteristics (collagenase, trypsin, clostripain, and proteases activity), and final islet yield were considered. Multivariate regression analysis showed only an independent association between SI and the concentration of collagenase (p = 0.01).
AB - We report the average insulin response to acute glucose measured by in vitro perifusion of pancreatic islets isolated from 80 consecutive human organs. Different perifusion parameters were considered [basal release, stimulation index (SI), time to peak, incremental area under the curve Δ- AUCa)], and the correlation among them was determined. SI positively correlated with A-AUCa (p <0.001, r = 0.80) while negatively with time to peak (p <0.05, r = -0.23). We also evaluated several variables of the isolation procedure that might affect responsiveness to glucose by human islets. Sex and age of pancreas donors, cold ischemia time, duration of the digestion, collagenase concentration, and lot characteristics (collagenase, trypsin, clostripain, and proteases activity), and final islet yield were considered. Multivariate regression analysis showed only an independent association between SI and the concentration of collagenase (p = 0.01).
KW - Insulin response
KW - Pancreatic islets
KW - Perifusion parameters
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M3 - Article
C2 - 10701499
AN - SCOPUS:0033384465
SN - 0963-6897
VL - 8
SP - 709
EP - 712
JO - Cell Transplantation
JF - Cell Transplantation
IS - 6
ER -