TY - JOUR
T1 - Lack of type 1 and type 2A myosin heavy chain isoforms in rat slow muscle regenerating during chronic nerve block
AU - Midrio, Menotti
AU - Danieli-Betto, Daniela
AU - Esposito, Alessandra
AU - Megighian, Aram
AU - Carraro, Ugo
AU - Catani, Claudia
AU - Rossini, Katia
PY - 1998
Y1 - 1998
N2 - The degeneration-regeneration process was induced by bupivacaine injection in innervated, denervated, and nerve-blocked rat soleus muscles. Nerve block was obtained by superfusion of the sciatic nerve with tetrodotoxin (TTX). Two weeks after bupivacaine injection, immunohistochemical and electrophoretical analyses showed the presence of type 1 myosin heavy chain (MHC) only in innervated regenerated muscles, type 2A in innervated and denervated, but not in TTX-paralyzed muscles, and type 2X under all experimental conditions. The presence of type 1 MHC in the innervated, and its absence in both denervated and TTX-paralyzed muscles were also verified immunohistochemically 1 week after bupivacaine injection. It is concluded that the nerve impulses play a determinant role in the expression of 1 and 2A MHC isoforms in the innervated regenerating muscle. The possible causes of the absence of the type 2A MHC isoform in the TTX-paralyzed muscles are discussed.
AB - The degeneration-regeneration process was induced by bupivacaine injection in innervated, denervated, and nerve-blocked rat soleus muscles. Nerve block was obtained by superfusion of the sciatic nerve with tetrodotoxin (TTX). Two weeks after bupivacaine injection, immunohistochemical and electrophoretical analyses showed the presence of type 1 myosin heavy chain (MHC) only in innervated regenerated muscles, type 2A in innervated and denervated, but not in TTX-paralyzed muscles, and type 2X under all experimental conditions. The presence of type 1 MHC in the innervated, and its absence in both denervated and TTX-paralyzed muscles were also verified immunohistochemically 1 week after bupivacaine injection. It is concluded that the nerve impulses play a determinant role in the expression of 1 and 2A MHC isoforms in the innervated regenerating muscle. The possible causes of the absence of the type 2A MHC isoform in the TTX-paralyzed muscles are discussed.
KW - Muscle differentiation
KW - Myosin heavy chain
KW - Nerve activity
KW - Neurotrophic factors
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U2 - 10.1002/(SICI)1097-4598(199802)21:2<226::AID-MUS10>3.0.CO;2-#
DO - 10.1002/(SICI)1097-4598(199802)21:2<226::AID-MUS10>3.0.CO;2-#
M3 - Article
C2 - 9466598
SN - 0148-639X
VL - 21
SP - 226
EP - 232
JO - Muscle and Nerve
JF - Muscle and Nerve
IS - 2
ER -