TY - CHAP
T1 - Labeling and tracking of human mesenchymal stem cells using near-infrared technology
AU - Armentero, Marie Therese
AU - Bossolasco, Patrizia
AU - Cova, Lidia
PY - 2013
Y1 - 2013
N2 - The recently developed near-infrared (NIR) light imaging technology combines low background noise with deep tissue penetration and readily allows imaging and tracking of NIR-labeled cells, following transplantation in small animal model of diseases. The real-time and longitudinal detection of grafted cells in vivo, as well as their rapid ex vivo localization, may further clarify graft interactions with the surrounding, in target and nontarget organs throughout the body, over time. The present chapter describes a protocol for (1) the efficient labeling of human mesenchymal stem cells (hMSCs) using a membrane intercalating dye, emitting in the NIR 815 nm spectrum; (2) the stereotaxic transplantation of NIR815-hMSCs in rodent model of Parkinson's disease; and (3) the longitudinal in vivo detection of the grafted cells and the subsequent ex vivo imaging in selected tissues.
AB - The recently developed near-infrared (NIR) light imaging technology combines low background noise with deep tissue penetration and readily allows imaging and tracking of NIR-labeled cells, following transplantation in small animal model of diseases. The real-time and longitudinal detection of grafted cells in vivo, as well as their rapid ex vivo localization, may further clarify graft interactions with the surrounding, in target and nontarget organs throughout the body, over time. The present chapter describes a protocol for (1) the efficient labeling of human mesenchymal stem cells (hMSCs) using a membrane intercalating dye, emitting in the NIR 815 nm spectrum; (2) the stereotaxic transplantation of NIR815-hMSCs in rodent model of Parkinson's disease; and (3) the longitudinal in vivo detection of the grafted cells and the subsequent ex vivo imaging in selected tissues.
KW - 6-Hydroxydopamine
KW - Cell imaging
KW - Cell therapy
KW - Intra-striatal injection
KW - Neurodegeneration
KW - Parkinson's disease
KW - Stem cell transplantation
UR - http://www.scopus.com/inward/record.url?scp=84883235125&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883235125&partnerID=8YFLogxK
U2 - 10.1007/7651-2013-21
DO - 10.1007/7651-2013-21
M3 - Chapter
C2 - 23640251
AN - SCOPUS:84883235125
SN - 9781627035583
VL - 1052
T3 - Methods in Molecular Biology
SP - 13
EP - 28
BT - Methods in Molecular Biology
ER -