Intercellular Ca2+ waves sustain coordinate insulin secretion in pig islets of Langerhans

F. Bertuzzi; D. Zacchetti; C. Berra; C. Socci; G. Pozza; A. E. Pontiroli; F. Grohovaz

doi:10.1016/0014-5793(95)01422-5

Intercellular Ca2+ waves sustain coordinate insulin secretion in pig islets of Langerhans

F. Bertuzzi, D. Zacchetti, C. Berra, C. Socci, G. Pozza, A. E. Pontiroli, F. Grohovaz

Research output: Contribution to journal › Article › peer-review

Abstract

Insulin release was investigated in parallel with changes in cytosolic calcium concentration, [Ca²⁺]_i, in pig islets stimulated by glucose. After two days in culture, glucose stimulation failed to induce insulin release, and caused limited [Ca²⁺]_i changes in few cells. After ten days, insulin response was partially restored and [Ca²⁺]_i recordings revealed a slow oscillatory activity of the whole islet. Slow oscillations appeared to be due to the average [Ca²⁺]_i variations resulting from the spreading of waves throughout the islet. These waves demonstrate the reestablishment of functional cell coupling, which appears to play a critical role in insulin release.

Original language	English
Pages (from-to)	21-25
Number of pages	5
Journal	FEBS Letters
Volume	379
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(95)01422-5
Publication status	Published - Jan 22 1996

Keywords

Ca oscillation
Ca wave
Fura-2
Insulin secretion
Islets of Langerhans

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0014-5793(95)01422-5

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abstract = "Insulin release was investigated in parallel with changes in cytosolic calcium concentration, [Ca2+]i, in pig islets stimulated by glucose. After two days in culture, glucose stimulation failed to induce insulin release, and caused limited [Ca2+]i changes in few cells. After ten days, insulin response was partially restored and [Ca2+]i recordings revealed a slow oscillatory activity of the whole islet. Slow oscillations appeared to be due to the average [Ca2+]i variations resulting from the spreading of waves throughout the islet. These waves demonstrate the reestablishment of functional cell coupling, which appears to play a critical role in insulin release.",

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AU - Bertuzzi, F.

AU - Zacchetti, D.

AU - Berra, C.

AU - Socci, C.

AU - Pozza, G.

AU - Pontiroli, A. E.

AU - Grohovaz, F.

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Intercellular Ca2+ waves sustain coordinate insulin secretion in pig islets of Langerhans

Abstract

Keywords

ASJC Scopus subject areas

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