Interaction of magnetic nanoparticles with U87MG cells studied by synchrotron radiation X-ray fluorescence techniques

A. Gianoncelli; P. Marmorato; J. Ponti; L. Pascolo; B. Kaulich; C. Uboldi; F. Rossi; D. Makovec; M. Kiskinova; G. Ceccone

doi:10.1002/xrs.2475

Interaction of magnetic nanoparticles with U87MG cells studied by synchrotron radiation X-ray fluorescence techniques

A. Gianoncelli, P. Marmorato, J. Ponti, L. Pascolo, B. Kaulich, C. Uboldi, F. Rossi, D. Makovec, M. Kiskinova, G. Ceccone

IRCCS pediatrico Burlo Garofolo

Research output: Contribution to journal › Article › peer-review

Abstract

Synchrotron radiation (SR) X-ray microscopy combined with X-ray fluorescence (XRF) microspectroscopy provides unique information that have pushed the frontiers of biological research, particularly when investigating intracellular mechanisms. This work reports an SR-XRF microspectroscopy investigation on the distribution and the potential toxicity of Fe₂O₃ and CoFe₂O₄ nanoparticles (NPs) in U87MG glioblastoma-astrocytoma cells. The U87MG cells exposed to NPs concentrations ranging from 5 to 250μg/ml for 24h were analyzed in order to monitor both morphological and chemical changes. The SR-XRF maps complemented with XRM absorption and phase contrast images have revealed different intracellular distribution patterns for the two nanoparticles types allowing different mechanism of toxicity to be deduced.

Original language	English
Pages (from-to)	316-320
Number of pages	5
Journal	X-Ray Spectrometry
Volume	42
Issue number	4
DOIs	https://doi.org/10.1002/xrs.2475
Publication status	Published - Jul 2013

ASJC Scopus subject areas

Spectroscopy

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abstract = "Synchrotron radiation (SR) X-ray microscopy combined with X-ray fluorescence (XRF) microspectroscopy provides unique information that have pushed the frontiers of biological research, particularly when investigating intracellular mechanisms. This work reports an SR-XRF microspectroscopy investigation on the distribution and the potential toxicity of Fe2O3 and CoFe2O4 nanoparticles (NPs) in U87MG glioblastoma-astrocytoma cells. The U87MG cells exposed to NPs concentrations ranging from 5 to 250μg/ml for 24h were analyzed in order to monitor both morphological and chemical changes. The SR-XRF maps complemented with XRM absorption and phase contrast images have revealed different intracellular distribution patterns for the two nanoparticles types allowing different mechanism of toxicity to be deduced.",

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AU - Gianoncelli, A.

AU - Marmorato, P.

AU - Ponti, J.

AU - Pascolo, L.

AU - Kaulich, B.

AU - Uboldi, C.

AU - Rossi, F.

AU - Makovec, D.

AU - Kiskinova, M.

AU - Ceccone, G.

PY - 2013/7

Y1 - 2013/7

N2 - Synchrotron radiation (SR) X-ray microscopy combined with X-ray fluorescence (XRF) microspectroscopy provides unique information that have pushed the frontiers of biological research, particularly when investigating intracellular mechanisms. This work reports an SR-XRF microspectroscopy investigation on the distribution and the potential toxicity of Fe2O3 and CoFe2O4 nanoparticles (NPs) in U87MG glioblastoma-astrocytoma cells. The U87MG cells exposed to NPs concentrations ranging from 5 to 250μg/ml for 24h were analyzed in order to monitor both morphological and chemical changes. The SR-XRF maps complemented with XRM absorption and phase contrast images have revealed different intracellular distribution patterns for the two nanoparticles types allowing different mechanism of toxicity to be deduced.

AB - Synchrotron radiation (SR) X-ray microscopy combined with X-ray fluorescence (XRF) microspectroscopy provides unique information that have pushed the frontiers of biological research, particularly when investigating intracellular mechanisms. This work reports an SR-XRF microspectroscopy investigation on the distribution and the potential toxicity of Fe2O3 and CoFe2O4 nanoparticles (NPs) in U87MG glioblastoma-astrocytoma cells. The U87MG cells exposed to NPs concentrations ranging from 5 to 250μg/ml for 24h were analyzed in order to monitor both morphological and chemical changes. The SR-XRF maps complemented with XRM absorption and phase contrast images have revealed different intracellular distribution patterns for the two nanoparticles types allowing different mechanism of toxicity to be deduced.

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Interaction of magnetic nanoparticles with U87MG cells studied by synchrotron radiation X-ray fluorescence techniques

Abstract

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