TY - JOUR
T1 - Innate and adaptive immunity in self-reported nonceliac gluten sensitivity versus celiac disease
AU - Di Sabatino, Antonio
AU - Giuffrida, Paolo
AU - Fornasa, Giulia
AU - Salvatore, Chiara
AU - Vanoli, Alessandro
AU - Naviglio, Samuele
AU - De Leo, Luigina
AU - Pasini, Alessandra
AU - De Amici, Mara
AU - Alvisi, Costanza
AU - Not, Tarcisio
AU - Rescigno, Maria
AU - Corazza, Gino Roberto
N1 - Copyright © 2016 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.
PY - 2016/7
Y1 - 2016/7
N2 - BACKGROUND: Immune mechanisms have been implicated in nonceliac gluten sensitivity (NCGS), a condition characterized by intestinal and/or extraintestinal symptoms caused by the ingestion of gluten in non-celiac/non-wheat allergic individuals.AIMS: We investigated innate and adaptive immunity in self-reported NCGS versus celiac disease (CD).METHODS: In the supernatants of ex vivo-cultured duodenal biopsies from 14 self-reported NCGS patients, 9 untreated and 10 treated CD patients, and 12 controls we detected innate cytokines - interleukin (IL)-15, tumor necrosis factor-α, IL-1β, IL-6, IL-12p70, IL-23, IL-27, IL-32α, thymic stromal lymphopoietin (TSLP), IFN-α-, adaptive cytokines - interferon (IFN)-γ, IL-17A, IL-4, IL-5, IL-10, IL-13-, chemokines - IL-8, CCL1, CCL2, CCL3, CCL4, CCL5, CXCL1, CXCL10-, granulocyte colony stimulating factor (G-CSF) and granulocyte-macrophage colony stimulating factor (GM-CSF).RESULTS: Mucosal innate and adaptive cytokines, chemokines and growth factors did not differ between self-reported NCGS, treated CD and controls. On the contrary, IL-6, IL-15, IL-27, IFN-α, IFN-γ, IL-17A, IL-23, G-CSF, GM-CSF, IL-8, CCL1 and CCL4 were significantly higher in untreated CD than in self-reported NCGS, treated CD and controls, while TSLP was significantly lower in untreated CD than in self-reported NCGS, treated CD and controls.CONCLUSION: In our hands, patients with self-reported NCGS showed no abnormalities of the mucosal immune response.
AB - BACKGROUND: Immune mechanisms have been implicated in nonceliac gluten sensitivity (NCGS), a condition characterized by intestinal and/or extraintestinal symptoms caused by the ingestion of gluten in non-celiac/non-wheat allergic individuals.AIMS: We investigated innate and adaptive immunity in self-reported NCGS versus celiac disease (CD).METHODS: In the supernatants of ex vivo-cultured duodenal biopsies from 14 self-reported NCGS patients, 9 untreated and 10 treated CD patients, and 12 controls we detected innate cytokines - interleukin (IL)-15, tumor necrosis factor-α, IL-1β, IL-6, IL-12p70, IL-23, IL-27, IL-32α, thymic stromal lymphopoietin (TSLP), IFN-α-, adaptive cytokines - interferon (IFN)-γ, IL-17A, IL-4, IL-5, IL-10, IL-13-, chemokines - IL-8, CCL1, CCL2, CCL3, CCL4, CCL5, CXCL1, CXCL10-, granulocyte colony stimulating factor (G-CSF) and granulocyte-macrophage colony stimulating factor (GM-CSF).RESULTS: Mucosal innate and adaptive cytokines, chemokines and growth factors did not differ between self-reported NCGS, treated CD and controls. On the contrary, IL-6, IL-15, IL-27, IFN-α, IFN-γ, IL-17A, IL-23, G-CSF, GM-CSF, IL-8, CCL1 and CCL4 were significantly higher in untreated CD than in self-reported NCGS, treated CD and controls, while TSLP was significantly lower in untreated CD than in self-reported NCGS, treated CD and controls.CONCLUSION: In our hands, patients with self-reported NCGS showed no abnormalities of the mucosal immune response.
KW - Journal Article
U2 - 10.1016/j.dld.2016.03.024
DO - 10.1016/j.dld.2016.03.024
M3 - Article
C2 - 27130911
SN - 1590-8658
VL - 48
SP - 745
EP - 752
JO - Digestive and Liver Disease
JF - Digestive and Liver Disease
IS - 7
ER -