Induction of myogenic differentiation by SDF-1 via CXCR4 and CXCR7 receptors

Roberta Melchionna; Anna Di Carlo; Roberta De Mori; Claudia Cappuzzello; Laura Barberi; Antonio Musarò; Chiara Cencioni; Nobutaka Fujii; Hirokazu Tamamura; Marco Crescenzi; Maurizio C. Capogrossi; Monica Napolitano; Antonia Germani

doi:10.1002/mus.21611

Induction of myogenic differentiation by SDF-1 via CXCR4 and CXCR7 receptors

Roberta Melchionna, Anna Di Carlo, Roberta De Mori, Claudia Cappuzzello, Laura Barberi, Antonio Musarò, Chiara Cencioni, Nobutaka Fujii, Hirokazu Tamamura, Marco Crescenzi, Maurizio C. Capogrossi, Monica Napolitano, Antonia Germani

Istituto Dermopatico dell'Immacolata , IDI

Research output: Contribution to journal › Article › peer-review

Abstract

The stromal cell-derived factor (SDF)-1/CXC receptor 4 (CXCR4) axis has been shown to play a role in skeletal muscle development, but its contribution to postnatal myogenesis and the role of the alternate SDF-1 receptor, CXC receptor 7 (CXCR7), are poorly characterized. Western blot analysis and real-time polymerase chain reaction (PCR) were performed to evaluate in vitro the effect of SDF-1 and CXCR4 and CXCR7 inhibition on myogenic differentiation. Proliferating myoblasts express CXCR4, CXCR7, and SDF-1; during myogenic differentiation, CXCR4 and CXCR7 levels are downregulated, and SDF-1 release is decreased. SDF-1 anticipates myosin heavy chain accumulation and myotube formation in both C2C12 myoblasts and satellite cells. Interestingly, inhibition of CXCR4 and CXCR7 signaling, either by drugs or RNA interfererence, blocks myogenic differentiation. Further, the CXCR4 antagonist, 4F-benzoyl-TN14003, inhibits myoblast cell cycle withdrawal and decreases the retinoblastoma gene (pRb) product accumulation in its hypophosphorylated form. Our experiments demonstrate that SDF-1 regulates myogenic differentiation via both CXCR4 and CXCR7 chemokine receptors.

Original language	English
Pages (from-to)	828-835
Number of pages	8
Journal	Muscle and Nerve
Volume	41
Issue number	6
DOIs	https://doi.org/10.1002/mus.21611
Publication status	Published - Jun 2010

Keywords

Chemokine
Myogenesis
Regeneration
Satellite cells
SDF-1

ASJC Scopus subject areas

Clinical Neurology
Cellular and Molecular Neuroscience
Physiology (medical)
Physiology

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10.1002/mus.21611

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title = "Induction of myogenic differentiation by SDF-1 via CXCR4 and CXCR7 receptors",

abstract = "The stromal cell-derived factor (SDF)-1/CXC receptor 4 (CXCR4) axis has been shown to play a role in skeletal muscle development, but its contribution to postnatal myogenesis and the role of the alternate SDF-1 receptor, CXC receptor 7 (CXCR7), are poorly characterized. Western blot analysis and real-time polymerase chain reaction (PCR) were performed to evaluate in vitro the effect of SDF-1 and CXCR4 and CXCR7 inhibition on myogenic differentiation. Proliferating myoblasts express CXCR4, CXCR7, and SDF-1; during myogenic differentiation, CXCR4 and CXCR7 levels are downregulated, and SDF-1 release is decreased. SDF-1 anticipates myosin heavy chain accumulation and myotube formation in both C2C12 myoblasts and satellite cells. Interestingly, inhibition of CXCR4 and CXCR7 signaling, either by drugs or RNA interfererence, blocks myogenic differentiation. Further, the CXCR4 antagonist, 4F-benzoyl-TN14003, inhibits myoblast cell cycle withdrawal and decreases the retinoblastoma gene (pRb) product accumulation in its hypophosphorylated form. Our experiments demonstrate that SDF-1 regulates myogenic differentiation via both CXCR4 and CXCR7 chemokine receptors.",

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author = "Roberta Melchionna and {Di Carlo}, Anna and {De Mori}, Roberta and Claudia Cappuzzello and Laura Barberi and Antonio Musar{\`o} and Chiara Cencioni and Nobutaka Fujii and Hirokazu Tamamura and Marco Crescenzi and Capogrossi, {Maurizio C.} and Monica Napolitano and Antonia Germani",

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AU - Melchionna, Roberta

AU - Di Carlo, Anna

AU - De Mori, Roberta

AU - Cappuzzello, Claudia

AU - Barberi, Laura

AU - Musarò, Antonio

AU - Cencioni, Chiara

AU - Fujii, Nobutaka

AU - Tamamura, Hirokazu

AU - Crescenzi, Marco

AU - Capogrossi, Maurizio C.

AU - Napolitano, Monica

AU - Germani, Antonia

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Induction of myogenic differentiation by SDF-1 via CXCR4 and CXCR7 receptors

Abstract

Keywords

ASJC Scopus subject areas

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