Induction of myogenic differentiation by SDF-1 via CXCR4 and CXCR7 receptors

Roberta Melchionna, Anna Di Carlo, Roberta De Mori, Claudia Cappuzzello, Laura Barberi, Antonio Musarò, Chiara Cencioni, Nobutaka Fujii, Hirokazu Tamamura, Marco Crescenzi, Maurizio C. Capogrossi, Monica Napolitano, Antonia Germani

Research output: Contribution to journalArticlepeer-review


The stromal cell-derived factor (SDF)-1/CXC receptor 4 (CXCR4) axis has been shown to play a role in skeletal muscle development, but its contribution to postnatal myogenesis and the role of the alternate SDF-1 receptor, CXC receptor 7 (CXCR7), are poorly characterized. Western blot analysis and real-time polymerase chain reaction (PCR) were performed to evaluate in vitro the effect of SDF-1 and CXCR4 and CXCR7 inhibition on myogenic differentiation. Proliferating myoblasts express CXCR4, CXCR7, and SDF-1; during myogenic differentiation, CXCR4 and CXCR7 levels are downregulated, and SDF-1 release is decreased. SDF-1 anticipates myosin heavy chain accumulation and myotube formation in both C2C12 myoblasts and satellite cells. Interestingly, inhibition of CXCR4 and CXCR7 signaling, either by drugs or RNA interfererence, blocks myogenic differentiation. Further, the CXCR4 antagonist, 4F-benzoyl-TN14003, inhibits myoblast cell cycle withdrawal and decreases the retinoblastoma gene (pRb) product accumulation in its hypophosphorylated form. Our experiments demonstrate that SDF-1 regulates myogenic differentiation via both CXCR4 and CXCR7 chemokine receptors.

Original languageEnglish
Pages (from-to)828-835
Number of pages8
JournalMuscle and Nerve
Issue number6
Publication statusPublished - Jun 2010


  • Chemokine
  • Myogenesis
  • Regeneration
  • Satellite cells
  • SDF-1

ASJC Scopus subject areas

  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)
  • Physiology


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