Increased platelet aggregation due to a plasma-aggregating activity: Identification of the responsible factors

G. G. Neri Serneri, R. Abbate, C. Mugnaini, G. F. Gensini

Research output: Contribution to journalArticlepeer-review


Platelet-poor plasmas (PPPs) from 52 out of 71 patients affected by different diseases and selected for increased amounts of circulating platelet aggregates, incubated at 37 °C for 30 min with control platelet-rich plasma (PRP; cross-matching test) induced the formation of platelet aggregates, stimulated the production of malondialdehyde and enhanced ADP-induced aggregation. 31 control PPPs were consistently negative at cross-matching test. Gel chromatography of plasmas on agarose 4% allowed the identification of four different fractions (A, B, C, D) provided with aggregating activity. Fraction A eluted at the void volume was to be identified with the von Willebrand factor, whereas B, C and D fractions, eluted at the same elution volumes as activated factor X were mainly composed of factor X (fraction B) and activated factor X (fractions C and D). Thrombin activity was absent in all the fractions provided with aggregating activity. Also, from control PPPs negative at cross-matching tests, the same fractions could be obtained, although of 5–10 times lower aggregating potency, thus explaining the negative cross-matching tests. These findings stress the role of some extraplatelet factors related to hypercoagulability in platelet hyperaggregability.

Original languageEnglish
Pages (from-to)141-156
Number of pages16
JournalPathophysiology of Haemostasis and Thrombosis
Issue number3
Publication statusPublished - 1980


  • Blood platelets
  • Hypercoagulability
  • Platelet aggregation

ASJC Scopus subject areas

  • Hematology
  • Physiology (medical)


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