TY - JOUR
T1 - Increased blood volume and CD34+CD38- progenitor cell recovery using a novel umbilical cord blood collection system
AU - Belvedere, Ornella
AU - Feruglio, Cristina
AU - Malangone, Walter
AU - Bonora, Maria L.
AU - Minisini, Alessandro M.
AU - Spizzo, Riccardo
AU - Donini, Annibale
AU - Sala, Pierguido
AU - De Anna, Dino
AU - Hilbert, David M.
AU - Degrassi, Alberto
PY - 2000
Y1 - 2000
N2 - A major problem with the use of umbilical cord/placental blood (UCB) is the limited blood volume that can be collected from a single donor. In this study, we evaluated a novel system for the collection of UCB and analyzed the kinetics of output of hematopoietic stem cells in the collected blood. Sequential UCB fractions were collected from 48 placentas by gravity following common procedures. When UCB flow was ended, collection was continued using the device. Nucleated cell (NC) density in each fraction was evaluated and the expression of CD34, CD38 and other hematopoietic markers was assessed by flow cytometry. The total collected volume was 60.9 ± 26.2 ml (mean ± SD, range 17-141.5). The device yield (volume collected using the device/total volume) was 26.5 ± 15.1%. No significant difference was observed in NC count in sequential fractions. A significant increase in CD34+ cell content in sequential fractions and a 2.07 ± 1.18-fold increase in the percentage of CD34+ cells in the last versus first fraction were observed. Furthermore, within the CD34+ population, the percentage of CD38- pluripotent stem cells in the first fraction was 3.24 ± 1.39, while in the last fraction it raised to 34.43 ± 22.62. Thus, at the end of a collection performed following current procedures, further blood rich in the most primitive progenitor cells can be recovered. Therefore, the optimization and standardization of collection procedures are required to obtain maximal recovery from each placenta and increase the percentage of UCB units suitable for clinical use.
AB - A major problem with the use of umbilical cord/placental blood (UCB) is the limited blood volume that can be collected from a single donor. In this study, we evaluated a novel system for the collection of UCB and analyzed the kinetics of output of hematopoietic stem cells in the collected blood. Sequential UCB fractions were collected from 48 placentas by gravity following common procedures. When UCB flow was ended, collection was continued using the device. Nucleated cell (NC) density in each fraction was evaluated and the expression of CD34, CD38 and other hematopoietic markers was assessed by flow cytometry. The total collected volume was 60.9 ± 26.2 ml (mean ± SD, range 17-141.5). The device yield (volume collected using the device/total volume) was 26.5 ± 15.1%. No significant difference was observed in NC count in sequential fractions. A significant increase in CD34+ cell content in sequential fractions and a 2.07 ± 1.18-fold increase in the percentage of CD34+ cells in the last versus first fraction were observed. Furthermore, within the CD34+ population, the percentage of CD38- pluripotent stem cells in the first fraction was 3.24 ± 1.39, while in the last fraction it raised to 34.43 ± 22.62. Thus, at the end of a collection performed following current procedures, further blood rich in the most primitive progenitor cells can be recovered. Therefore, the optimization and standardization of collection procedures are required to obtain maximal recovery from each placenta and increase the percentage of UCB units suitable for clinical use.
KW - CD34
KW - CD38
KW - Device
KW - Hematopoietic stem cells
KW - Umbilical cord blood
KW - Umbilical cord blood collection
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M3 - Article
C2 - 10924090
AN - SCOPUS:0033867240
SN - 1066-5099
VL - 18
SP - 245
EP - 251
JO - Stem Cells
JF - Stem Cells
IS - 4
ER -