TY - JOUR
T1 - 'In vivo' studies on the pathophysiological mechanism of Vibrio parahaemolyticus TDH+-induced secretion
AU - Baffone, W.
AU - Casaroli, A.
AU - Campana, R.
AU - Citterio, B.
AU - Vittoria, E.
AU - Pierfelici, L.
AU - Donelli, G.
PY - 2005/2
Y1 - 2005/2
N2 - The thermostable direct haemolysin (TDH) is considered to be the major virulence factors of Vibrio parahaemolyticus; however, poor information is available about its mechanism of action. In our study we examined the capacity of two V. parahaemolyticus TDH-producers (strains 2067 and 3305) to induce fluid secretion in rat ileal loop and to reveal the role of calcium ions (Ca 2+), calmodulin (CaM), and protein kinase C (PKC) in V. parahaemolyticus TDH+-induced fluid secretion. The results show that V. parahaemolyticus TDH+ strains were able to induce secretion in small intestine; on the contrary, this ability was not evidenced in the V. parahaemolyticus TDH- strain used as negative control. The data suggest an enterotoxic activity of haemolysin. Calcium ionophore A23187 and 1-verapamil (calcium channel blocker), when injected alone, induced fluid accumulation in the control loops. A further increase in fluid accumulation (P+ strains and a significant decrease (P2+ appears to be an important messenger involved in the stimulation of intestinal secretion, contrary to PKC and calmodulin which do not appear to have any role.
AB - The thermostable direct haemolysin (TDH) is considered to be the major virulence factors of Vibrio parahaemolyticus; however, poor information is available about its mechanism of action. In our study we examined the capacity of two V. parahaemolyticus TDH-producers (strains 2067 and 3305) to induce fluid secretion in rat ileal loop and to reveal the role of calcium ions (Ca 2+), calmodulin (CaM), and protein kinase C (PKC) in V. parahaemolyticus TDH+-induced fluid secretion. The results show that V. parahaemolyticus TDH+ strains were able to induce secretion in small intestine; on the contrary, this ability was not evidenced in the V. parahaemolyticus TDH- strain used as negative control. The data suggest an enterotoxic activity of haemolysin. Calcium ionophore A23187 and 1-verapamil (calcium channel blocker), when injected alone, induced fluid accumulation in the control loops. A further increase in fluid accumulation (P+ strains and a significant decrease (P2+ appears to be an important messenger involved in the stimulation of intestinal secretion, contrary to PKC and calmodulin which do not appear to have any role.
KW - Pathophysiological mechanism
KW - TDH
KW - Vibrio parahaemolyticus
UR - http://www.scopus.com/inward/record.url?scp=14644387505&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=14644387505&partnerID=8YFLogxK
U2 - 10.1016/j.micpath.2004.11.001
DO - 10.1016/j.micpath.2004.11.001
M3 - Article
C2 - 15748815
AN - SCOPUS:14644387505
SN - 0882-4010
VL - 38
SP - 133
EP - 137
JO - Microbial Pathogenesis
JF - Microbial Pathogenesis
IS - 2-3
ER -