In vivo single voxel 1H MRS of glial brain tumors: Correlation with tissue histology and in vitro MRS

G. Barbarella, R. Ricci, G. Pirini, V. Tugnoli, M. R. Tosi, A. Bertoluzza, F. Calbucci, M. Leonardi, C. Trevisan, V. Eusebi

Research output: Contribution to journalArticlepeer-review


Eighteen patients affected by glial brain tumors were examined by localized single voxel magnetic resonance spectroscopy (1H MRS). For all patients histological diagnosis was obtained after stereotactic biopsy or surgical removal of the tumor. Histological nuclear atypia, tumor cellularity, necrosis, vascular proliferation and percentage of Ki-67 positive cells in the total cell populations were evaluated. It was found that some of the in vivo 1H MRS parameters were related to the histological features of the tumor. In particular, the Cho(i)/Cr(i) metabolite ratio obtained from the neoplastic tissues paralleled the Ki-67 cell proliferation index. For some of the patients 1H and 13C in vitro high resolution magnetic spectroscopy of surgical specimens was also carried out. 1H MRS of aqueous extracts from different biopsy specimens of the same tumor furnished fine biochemical information on intratumor heterogeneity. The degree of histological vascular proliferation of the tumor was correlated to the presence of cholesteryl esters in the 13C spectrum of the lipid fraction of surgical specimens.

Original languageEnglish
Pages (from-to)461-468
Number of pages8
JournalInternational Journal of Oncology
Issue number2
Publication statusPublished - Feb 1998


  • Brain tumors
  • Magnetic resonance spectroscopy
  • Tissue histology

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


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