In vivo single voxel 1H MRS of glial brain tumors: Correlation with tissue histology and in vitro MRS

Eighteen patients affected by glial brain tumors were examined by localized single voxel magnetic resonance spectroscopy (¹H MRS). For all patients histological diagnosis was obtained after stereotactic biopsy or surgical removal of the tumor. Histological nuclear atypia, tumor cellularity, necrosis, vascular proliferation and percentage of Ki-67 positive cells in the total cell populations were evaluated. It was found that some of the in vivo ¹H MRS parameters were related to the histological features of the tumor. In particular, the Cho(i)/Cr(i) metabolite ratio obtained from the neoplastic tissues paralleled the Ki-67 cell proliferation index. For some of the patients ¹H and ¹³C in vitro high resolution magnetic spectroscopy of surgical specimens was also carried out. ¹H MRS of aqueous extracts from different biopsy specimens of the same tumor furnished fine biochemical information on intratumor heterogeneity. The degree of histological vascular proliferation of the tumor was correlated to the presence of cholesteryl esters in the ¹³C spectrum of the lipid fraction of surgical specimens.