TY - JOUR
T1 - In vitro evaluation of bio-functional performances of Ghimas titanium implants
AU - Gorrieri, O.
AU - Fini, M.
AU - Kyriakidou, K.
AU - Zizzi, A.
AU - Mattioli-Belmonte, Monica
AU - Castaldo, P.
AU - De Cristofaro, A.
AU - Natali, D.
AU - Pugnaloni, A.
AU - Biagini, G.
PY - 2006/10
Y1 - 2006/10
N2 - Titanium is the most widely used material for dental implants. The natural formation, in presence of oxygen, of different oxide films (passivation films) is correlated to titanium implant biocompatibility resistance to corrosion and is responsible for implant bacteriostatic action. Surface roughness is another surface property of Ti-implants that, affecting implant-to-bone contact, improves integration. In the present study data concerning composition, surface roughness and biocompatibility of Ghimas implants and mini-implants undergoing sandblasting with Calcium Magnesium Carbonate (CaMg(CO3)2) are reported. AFM, SEM/EDX, XRD analyses and morphofunctional tests (MTT and ALP) were performed. Cell actin cytoskeletal modification (fluorescence phalloidin staining) was also observed with confocal laser microscopy (CL SM). Data related to surface geometry and chemical properties, associated with evidence of high purity of all the tested materials (XRD and EDX), highlighted the elevated biocompatibility of tested implants and mini-implants. CLSM investigation confirmed osteoblast features of an active cell behavior able to fit cell to chemico-mechanical stimuli present at the bone/ implant interface and suggests an effective implant/ alveolar bone integration in vivo.
AB - Titanium is the most widely used material for dental implants. The natural formation, in presence of oxygen, of different oxide films (passivation films) is correlated to titanium implant biocompatibility resistance to corrosion and is responsible for implant bacteriostatic action. Surface roughness is another surface property of Ti-implants that, affecting implant-to-bone contact, improves integration. In the present study data concerning composition, surface roughness and biocompatibility of Ghimas implants and mini-implants undergoing sandblasting with Calcium Magnesium Carbonate (CaMg(CO3)2) are reported. AFM, SEM/EDX, XRD analyses and morphofunctional tests (MTT and ALP) were performed. Cell actin cytoskeletal modification (fluorescence phalloidin staining) was also observed with confocal laser microscopy (CL SM). Data related to surface geometry and chemical properties, associated with evidence of high purity of all the tested materials (XRD and EDX), highlighted the elevated biocompatibility of tested implants and mini-implants. CLSM investigation confirmed osteoblast features of an active cell behavior able to fit cell to chemico-mechanical stimuli present at the bone/ implant interface and suggests an effective implant/ alveolar bone integration in vivo.
KW - Actin cytoskeleton
KW - AFM roughness profile
KW - Cell behavior
KW - Confocal laser microscopy
KW - SEM
KW - Titanium implants
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M3 - Article
C2 - 17211823
AN - SCOPUS:33845867131
SN - 0391-3988
VL - 29
SP - 1012
EP - 1020
JO - International Journal of Artificial Organs
JF - International Journal of Artificial Organs
IS - 10
ER -