In vitro analysis of low-level laser irradiation on human osteoblast-like cells proliferation

Nora Bloise, Enrica Saino, Francesca Bragheri, Paolo Minzioni, Ilaria Cristiani, Marcello Imbriani, Livia Visai

Research output: Chapter in Book/Report/Conference proceedingConference contribution


The objective of this study was to examine the in vitro effect of a single or a multiple doses of low-level laser irradiation (LLLI) on proliferation of the human osteosarcoma cell line, SAOS-2. SAOS-2 cells were divided in five groups and exposed to LLLI (659 nm diode laser; 11 mW power output): group I as a control (dark), group II exposed to a single laser dose of 1 J/cm2, group III irradiated with a single dose of 3 J/cm2, and group IV and V exposed for three consecutive days to 1 or 3 J/cm2, respectively. Cellular proliferation was assessed daily up to 7 days of culturing. The obtained results showed an increase in proliferative capacity of SAOS-2 cells during the first 96 h of culturing time in once-irradiated cells, as compared to control cells. Furthermore, a significantly higher proliferation in the group IV and V was detected if compared to a single dose or to control group after 96 h and 7 days. In conclusion, the effect of the single dose on cell proliferation was transitory and repeated irradiations were necessary to observe a strong enhancement of SAOS-2 growth. As a future perspective, we would like to determine the potential of LLLI as a new approach for promoting bone regeneration onto biomaterials.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Publication statusPublished - 2011
EventMedical Laser Applications and Laser-Tissue Interactions V - Munich, Germany
Duration: May 24 2011May 26 2011


OtherMedical Laser Applications and Laser-Tissue Interactions V


  • Cell Proliferation
  • Low-Level Laser Irradiation (LLLI)
  • SAOS-2 human osteoblast-like cells

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


Dive into the research topics of 'In vitro analysis of low-level laser irradiation on human osteoblast-like cells proliferation'. Together they form a unique fingerprint.

Cite this