In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering

Andrea Peloso, Antonio Citro, Valeria Corradetti, Szandra Brambilla, Graziano Oldani, Francesco Calabrese, Tommaso Dominioni, Marcello Maestri, Lorenzo Cobianchi

Research output: Contribution to journalArticlepeer-review


Whole-organ decellularization is recently gaining interest in the transplantation field as strategy to obtain acellular scaffold only composed by extracellular matrix. These structures, that still remain organ-specific in terms of biological cues and tridimensional morphology could be then recellularized with patient's autologous cells. The final result should be a nwe transplantable autologous organ that should by-pass, at the same time, the problem of organ shortage and secondly the consequences related to the immunosuppression need. Herein we describe the protocol to manufacture a whole-organ transplantable rat kidney scaffold by a dual-detergent (Triton X-100 and SDS) arterial peristaltic perfusion. Final results show whole-renal acellular scaffold with contextual preservation of tridimensional architecture and biological properties deriving from the extracellular matrix composition.

Original languageEnglish
Pages (from-to)103-110
Number of pages8
JournalMethods in molecular biology (Clifton, N.J.)
Publication statusPublished - Jan 1 2018


  • Extracellular matrix
  • Kidney scaffold
  • Kidney transplantation
  • Organ bioengineering
  • Rat model
  • Regenerative medicine

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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