Abstract
The chemokine receptor CXCR3 is involved in cell trafficking dysregulation associated with several inflammatory conditions, including autoimmune and viral diseases. Downregulation of CXCR3, through binding with its ligand CXCL111(I-TAC), represents a key mechanism in lymphocyte recruitment. Determination of circulating I-TAC can provide useful information in the investigation of inflammatory/infectious conditions. The existing commercial kit does not measure CXCL11/I-TAC in complex matrices, such as human plasma and serum, as reliably as in in vitro-generated cell culture supernatants. We here describe means which lead to an improvement of CXCL11/I-TAC measurement in human plasma and serum.
| Original language | English |
|---|---|
| Pages (from-to) | 125-128 |
| Number of pages | 4 |
| Journal | New Microbiologica |
| Volume | 32 |
| Issue number | 1 |
| Publication status | Published - Jan 2009 |
Keywords
- Cell trafficking
- Chemokine receptors
- Chemokines
- CXCL11/I-TAC
- CXCR3
- ELISA
ASJC Scopus subject areas
- Microbiology (medical)