Improved avidin-biotin-peroxidase complex (ABC) staining

Giorgio Cattoretti, Emilio Berti, Raffaella Schiró, Lucia D'Amato, Costante Valeggio, Franco Rilke

Research output: Contribution to journalArticlepeer-review


A considerable intensification of the avidin-biotin-peroxidase complex staining system (ABC) was obtained by sequentially overlaying the sections to be immunostained with an avidin-rich and a biotin-rich complex. Each sequential addition contributed to the deposition of horseradish peroxidase on the immunostained site and allowed the subsequent binding of a complementary complex. With this technique a higher dilution of the antisera could be used and minute amounts of antigen masked by the fixative could be demonstrated on paraffin sections.

Original languageEnglish
Pages (from-to)75-80
Number of pages6
JournalHistochemical Journal
Issue number2
Publication statusPublished - Feb 1988

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology


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