TY - JOUR
T1 - Importance of surveillance of new delhi metallo-beta-lactamase klebsiella pneumoniae
T2 - Molecular characterization and clonality of strains isolated in the lazio region, italy
AU - Spaziante, Martina
AU - Venditti, Carolina
AU - Butera, Ornella
AU - Messina, Francesco
AU - Di Caro, Antonino
AU - Tonziello, Gilda
AU - Lanini, Simone
AU - Cataldo, Maria Adriana
AU - Puro, Vincenzo
N1 - Funding Information:
This research was funded by Ministero della salute - Ricerca Corrente IRCCS-Linea 1. Progetto 3.
Funding Information:
We gratefully acknowledge the colleagues of the Infectious Diseases Prophylaxis Units and of the Public Health and Hygiene Services (SISP) of the Local Health Services (ASL) of Lazio, the microbiology laboratories of participant hospitals as well as all the staff involved in the structures that reported the cases, for the activity carried out to the surveillance and control of invasive CRE infections in the Lazio region. Funding This research was funded by Ministero della salute-Ricerca Corrente IRCCS-Linea 1. Progetto 3.
Publisher Copyright:
© 2021 Spaziante et al.
PY - 2021/9/8
Y1 - 2021/9/8
N2 - Introduction: New Delhi metallo-β-lactamase producing Klebsiella pneumoniae (NDM-Kpn) strains have been causing healthcare-associated infections worldwide. The aim of this study was to describe the molecular mechanisms of antimicrobial resistance and to analyze the clonality of NDM-Kpn isolates collected between January 2019 and June 2020 from patients admitted to hospitals from the Lazio region, Italy. Methods: We performed a retrospective cohort study. Whole-genome sequencing (WGS) was performed on all NDM-Kpn strains; clonality and genetic relationships were further investigated. Results: During the surveillance period, 17 NDM-Kpn isolates were obtained from 17 patients admitted to seven different hospitals. Eight different sequence types (STs) were detected: ST147 (n = 4), ST383 (n = 4), ST15 (n = 3), ST11 (n = 2), ST17 (n = 1), ST29 (n = 1), ST307 (n = 1) and the newly identified ST4853 (n = 1). Genetic relationships were further investigated by the WGS-based core genome MLST (cgMLST) scheme, and 5 cluster types (CTs) were identified. Whereas a substantial overall heterogeneity among isolates was detected (8 different STs were identified out of 17 isolates), the strains within each cluster showed a very high level of genome similarity. Discussion: Our study highlights the key role of surveillance, which allowed taking a picture of a part of the NDM-Kpn strains circulating in Italy, adding further insight into their molecular features.
AB - Introduction: New Delhi metallo-β-lactamase producing Klebsiella pneumoniae (NDM-Kpn) strains have been causing healthcare-associated infections worldwide. The aim of this study was to describe the molecular mechanisms of antimicrobial resistance and to analyze the clonality of NDM-Kpn isolates collected between January 2019 and June 2020 from patients admitted to hospitals from the Lazio region, Italy. Methods: We performed a retrospective cohort study. Whole-genome sequencing (WGS) was performed on all NDM-Kpn strains; clonality and genetic relationships were further investigated. Results: During the surveillance period, 17 NDM-Kpn isolates were obtained from 17 patients admitted to seven different hospitals. Eight different sequence types (STs) were detected: ST147 (n = 4), ST383 (n = 4), ST15 (n = 3), ST11 (n = 2), ST17 (n = 1), ST29 (n = 1), ST307 (n = 1) and the newly identified ST4853 (n = 1). Genetic relationships were further investigated by the WGS-based core genome MLST (cgMLST) scheme, and 5 cluster types (CTs) were identified. Whereas a substantial overall heterogeneity among isolates was detected (8 different STs were identified out of 17 isolates), the strains within each cluster showed a very high level of genome similarity. Discussion: Our study highlights the key role of surveillance, which allowed taking a picture of a part of the NDM-Kpn strains circulating in Italy, adding further insight into their molecular features.
KW - Carbapenem-resistant klebsiella pneumoniae
KW - Healthcare-associated infections
KW - New delhi metallo-β-lactamase
KW - Surveillance
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U2 - 10.2147/IDR.S318717
DO - 10.2147/IDR.S318717
M3 - Article
AN - SCOPUS:85114873788
SN - 1178-6973
VL - 14
SP - 3659
EP - 3665
JO - Infection and Drug Resistance
JF - Infection and Drug Resistance
ER -