Immunoglobulin and T cell receptor gene rearrangements and in situ immunophenotyping in lymphoproliferative disorders

Antonino Carbone, Valli De Re, Annunziata Gloghini, Rachele Volpe, Manuela Tavian, Umberto Tirelli, Silvio Monfardini, Mauro Boiocchi

Research output: Contribution to journalArticlepeer-review


We investigated for rearrangements of the immunoglobulin (Ig) heavy and light chain genes and of the T cell receptor γ (TCRT γ) and β (TCr β) genes 45 biopsy samples from a variety of lymphoproliferative disorders. They were diagnosed histopathologically and immunophenotypically as non-Hodgkin's lymphomas (NHLs) of the B cell type (19 cases), NHLs of the T cell type (3 cases), NHLs of "undetermined" cell type (3 cases), atypical lymphoid proliferation (1 case) and AIDS-related lymphadenopathies with florid polyclonal follicular hyperplasia (19 cases). A monoclonal proliferation of B cells was shown by DNA analysis in all 19 B cell NHLs. In two immunohistologically determined T cell NHLs (both diagnosed as mycosis fungoides) the cells had rearrangements of TCr β gene, whereas in the third case (lymphoblastic NHL) the cells had rearrangements of Ig heavy chain and TCr γ and TCr β genes. None of the B cell NHLs exhibited TCr γand TCr β gene rearrangement bands. All the "undetermined" cell NHLs demonstrated rearrangements of Ig heavy chain gene associated with the germ line TCr γand TCr β genes; in two cases light chain gene rearrangements were also found. The atypical lymphoid proliferation, in which the differential diagnosis was between a reactive or malignant process, and two out of 19 cases of florid polyclonal follicular hyperplasia showed a clonal B cell population by DNA analysis. This study indicates that there was a strong correlation between the rearrangements of specific genes and the immunophenotype of the NHL; moreover, DNA analysis of tissue biopsy specimens from phenotypically "undetermined" cell NHLs and from equivocal lymphoid proliferation using Ig and TCR gene probes yelded an answer in the cases analyzed. The significance of clonal B cell expansions found in two AIDS-related lymphadenopathies should be interpreted with caution.

Original languageEnglish
Pages (from-to)223-230
Number of pages8
JournalVirchows Archiv A Pathological Anatomy and Histopathology
Issue number3
Publication statusPublished - May 1989


  • AIDS
  • DNA analysis
  • Gene rearrangements
  • Histopathology
  • Immunohistology
  • Malignant lymphoma
  • Molecular hybridization

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy


Dive into the research topics of 'Immunoglobulin and T cell receptor gene rearrangements and in situ immunophenotyping in lymphoproliferative disorders'. Together they form a unique fingerprint.

Cite this