Immune history shapes specificity of pandemic H1N1 influenza antibody responses

Yang Li, Jaclyn L. Myers, David L. Bostick, Colleen B. Sullivan, Jonathan Madara, Susanne L. Linderman, Qin Liu, Donald M. Carter, Jens Wrammert, Susanna Esposito, Nicola Principi, Joshua B. Plotkin, Ted M. Ross, Rafi Ahmed, Patrick C. Wilson, Scott E. Hensley

Research output: Contribution to journalArticlepeer-review


Human antibody responses against the 2009 pandemic H1N1 (pH1N1) virus are predominantly directed against conserved epitopes in the stalk and receptor-binding domain of the hemagglutinin (HA) protein. This is in stark contrast to pH1N1 antibody responses generated in ferrets, which are focused on the variable Sa antigenic site of HA. Here, we show that most humans born between 1983 and 1996 elicited pH1N1 antibody responses that are directed against an epitope near the HA receptor-binding domain. Importantly, most individuals born before 1983 or after 1996 did not elicit pH1N1 antibodies to this HA epitope. The HAs of most seasonal H1N1 (sH1N1) viruses that circulated between 1983 and 1996 possess acritical K133 amino acid in this HA epitope, whereas this amino acid is either mutated ordeleted in most sH1N1 viruses circulating before 1983 or after 1996. We sequentially infected ferrets with a 1991 sH1N1 virus and then a pH1N1 virus. Sera isolated from these animals were directed against the HA epitope involving amino acid K133. These data suggest that the specificity of pH1N1 antibody responses can be shifted to epitopes near the HA receptor-binding domain after sequential infections with sH1N1 and pH1N1 viruses that share homology in this region.

Original languageEnglish
Pages (from-to)1493-1500
Number of pages8
JournalJournal of Experimental Medicine
Issue number8
Publication statusPublished - 2013

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy


Dive into the research topics of 'Immune history shapes specificity of pandemic H1N1 influenza antibody responses'. Together they form a unique fingerprint.

Cite this