TY - JOUR
T1 - Immune characterization of long pentraxin 3 in pigs infected with influenza virus
AU - Crisci, Elisa
AU - Fraile, Lorenzo
AU - Valentino, Sonia
AU - Martínez-Guinó, Laura
AU - Bottazzi, Barbara
AU - Mantovani, Alberto
AU - Montoya, Maria
PY - 2014/1/10
Y1 - 2014/1/10
N2 - Long pentraxin 3 (PTX3) is a conserved pattern-recognition secreted protein and a host-defence-related component of the humoral innate immune system. The aim of the present study was to characterize swine PTX3 (SwPTX3) protein expression in influenza virus infected pigs. First, we performed in silico studies to evaluate the cross-reactivity of PTX3 human antibodies against SwPTX3. Secondly, we used in vitro analysis to detect SwPTX3 presence in swine bone marrow dendritic cells (SwBMDC) upon stimulation with different agents by Western blot and immunofluorescence. Finally, the levels of SwPTX3 were assessed in experimental infection of pigs with different strains of influenza virus. This is a novel study where the expression of SwPTX3 was evaluated in the context of a pathogen infection. The initial characterization of SwPTX3 in influenza virus infected pigs contributes to understand the role of PTX proteins in the immune response.
AB - Long pentraxin 3 (PTX3) is a conserved pattern-recognition secreted protein and a host-defence-related component of the humoral innate immune system. The aim of the present study was to characterize swine PTX3 (SwPTX3) protein expression in influenza virus infected pigs. First, we performed in silico studies to evaluate the cross-reactivity of PTX3 human antibodies against SwPTX3. Secondly, we used in vitro analysis to detect SwPTX3 presence in swine bone marrow dendritic cells (SwBMDC) upon stimulation with different agents by Western blot and immunofluorescence. Finally, the levels of SwPTX3 were assessed in experimental infection of pigs with different strains of influenza virus. This is a novel study where the expression of SwPTX3 was evaluated in the context of a pathogen infection. The initial characterization of SwPTX3 in influenza virus infected pigs contributes to understand the role of PTX proteins in the immune response.
KW - DC
KW - Influenza virus
KW - Pigs
KW - PTX3
UR - http://www.scopus.com/inward/record.url?scp=84890803010&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84890803010&partnerID=8YFLogxK
U2 - 10.1016/j.vetmic.2013.10.004
DO - 10.1016/j.vetmic.2013.10.004
M3 - Article
C2 - 24238985
AN - SCOPUS:84890803010
SN - 0378-1135
VL - 168
SP - 185
EP - 192
JO - Veterinary Microbiology
JF - Veterinary Microbiology
IS - 1
ER -