Immobilization of mushroom tyrosinase on controlled pore glass: Effect of chemical modification

Anna Maria Girelli; Enrico Mattei; Antonella Messina; Domenica Papaleo

doi:10.1016/j.snb.2007.01.035

Immobilization of mushroom tyrosinase on controlled pore glass: Effect of chemical modification

Anna Maria Girelli, Enrico Mattei, Antonella Messina, Domenica Papaleo

Fondazione IRCCS Istituto Nazionale dei Tumori

Research output: Contribution to journal › Article › peer-review

Abstract

Mushroom tyrosinase immobilization on controlled pore glass with three different functional groups like alkylamino, carboxyl and arylamino is performed. The effect of the alkylamino-CPG arm extension on enzymatic stability and catalytic activity is also investigated. The results expressed in terms of ligand coupling, % yield and kinetic parameters, like the apparent K_m ^app and V_max ^app and the inherent K_m ^inh and V_max ^inh values demonstrate the best performance of tyrosinase immobilized on alkylammino-CPGs.

Original language	English
Pages (from-to)	48-54
Number of pages	7
Journal	Sensors and Actuators, B: Chemical
Volume	125
Issue number	1
DOIs	https://doi.org/10.1016/j.snb.2007.01.035
Publication status	Published - Jul 16 2007

Keywords

Bioreactor
Controlled pore glass
Kinetic parameters
Tyrosinase immobilization

ASJC Scopus subject areas

Analytical Chemistry
Electrochemistry
Electrical and Electronic Engineering

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10.1016/j.snb.2007.01.035

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title = "Immobilization of mushroom tyrosinase on controlled pore glass: Effect of chemical modification",

abstract = "Mushroom tyrosinase immobilization on controlled pore glass with three different functional groups like alkylamino, carboxyl and arylamino is performed. The effect of the alkylamino-CPG arm extension on enzymatic stability and catalytic activity is also investigated. The results expressed in terms of ligand coupling, % yield and kinetic parameters, like the apparent Km app and Vmax app and the inherent Km inh and Vmax inh values demonstrate the best performance of tyrosinase immobilized on alkylammino-CPGs.",

keywords = "Bioreactor, Controlled pore glass, Kinetic parameters, Tyrosinase immobilization",

author = "Girelli, {Anna Maria} and Enrico Mattei and Antonella Messina and Domenica Papaleo",

year = "2007",

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T1 - Immobilization of mushroom tyrosinase on controlled pore glass

T2 - Effect of chemical modification

AU - Girelli, Anna Maria

AU - Mattei, Enrico

AU - Messina, Antonella

AU - Papaleo, Domenica

PY - 2007/7/16

Y1 - 2007/7/16

N2 - Mushroom tyrosinase immobilization on controlled pore glass with three different functional groups like alkylamino, carboxyl and arylamino is performed. The effect of the alkylamino-CPG arm extension on enzymatic stability and catalytic activity is also investigated. The results expressed in terms of ligand coupling, % yield and kinetic parameters, like the apparent Km app and Vmax app and the inherent Km inh and Vmax inh values demonstrate the best performance of tyrosinase immobilized on alkylammino-CPGs.

AB - Mushroom tyrosinase immobilization on controlled pore glass with three different functional groups like alkylamino, carboxyl and arylamino is performed. The effect of the alkylamino-CPG arm extension on enzymatic stability and catalytic activity is also investigated. The results expressed in terms of ligand coupling, % yield and kinetic parameters, like the apparent Km app and Vmax app and the inherent Km inh and Vmax inh values demonstrate the best performance of tyrosinase immobilized on alkylammino-CPGs.

KW - Bioreactor

KW - Controlled pore glass

KW - Kinetic parameters

KW - Tyrosinase immobilization

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M3 - Article

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EP - 54

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

IS - 1

ER -

Immobilization of mushroom tyrosinase on controlled pore glass: Effect of chemical modification

Abstract

Keywords

ASJC Scopus subject areas

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