TY - JOUR
T1 - IFNβ bioavailability in multiple sclerosis patients
T2 - MxA versus antibody-detecting assays
AU - Capra, Ruggero
AU - Sottini, Alessandra
AU - Cordioli, Cinzia
AU - Serana, Federico
AU - Chiarini, Marco
AU - Caimi, Luigi
AU - Padovani, Alessandro
AU - Bergamaschi, Roberto
AU - Imberti, Luisa
PY - 2007/9
Y1 - 2007/9
N2 - Anti-IFNβ antibodies are related to IFNβ bioavailability loss in multiple sclerosis. We investigated the reliability of radioimmunoprecipitation and cytopathic assays in detecting binding (BAbs) and neutralizing (NAbs) antibodies and the correlation of these antibodies to MxA mRNA production. Eleven percent of IFNβ-treated patients showed a lack of MxA induction, with an inverse correlation between MxA mRNA and the presence of BAbs and NAbs. Some patients had contemporary MxA induction in the presence of high NAb titres, thus calling into question the reliability of cytopathic assay. Since anti-IFNβ antibodies well correlated with MxA induction loss, MxA assay is an appropriate test to determine IFNβ bioavailability.
AB - Anti-IFNβ antibodies are related to IFNβ bioavailability loss in multiple sclerosis. We investigated the reliability of radioimmunoprecipitation and cytopathic assays in detecting binding (BAbs) and neutralizing (NAbs) antibodies and the correlation of these antibodies to MxA mRNA production. Eleven percent of IFNβ-treated patients showed a lack of MxA induction, with an inverse correlation between MxA mRNA and the presence of BAbs and NAbs. Some patients had contemporary MxA induction in the presence of high NAb titres, thus calling into question the reliability of cytopathic assay. Since anti-IFNβ antibodies well correlated with MxA induction loss, MxA assay is an appropriate test to determine IFNβ bioavailability.
KW - BAbs
KW - Bioavailability
KW - Interferons
KW - Multiple sclerosis
KW - MxA
KW - NAbs
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UR - http://www.scopus.com/inward/citedby.url?scp=34548590673&partnerID=8YFLogxK
U2 - 10.1016/j.jneuroim.2007.06.006
DO - 10.1016/j.jneuroim.2007.06.006
M3 - Article
C2 - 17619058
AN - SCOPUS:34548590673
SN - 0165-5728
VL - 189
SP - 102
EP - 110
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1-2
ER -