IFN-γ gene transfer restores HLA-class I expression and MAGE-3 antigen presentation to CTL in HLA-deficient small cell lung cancer

In this study, we have analyzed the possibility of inducing T cell responses against small cell lung cancer (SCLC), a still incurable tumor, by cytokine gene transfer approaches. By RT-PCR analysis most SCLC expressed the CTL-defined tumor antigens MAGE-3 (10/11), MAGE-1 (7/11) and less frequently BAGE (4/11) and GAGE 1,2 (4/11). Although the surface expression of HLA class 1 molecules was low on most SCLC cell lines displaying the A2 HLA class 1 allele, involved in MAGE-3 antigen presentation to CTL, were stably transfected with the IFN-γ gene (alone or co-transfected with IL-2). IFN-γ-transfected cell displayed a clearcut increase in expression of HLA class 1 and β₂ microglobulin at both protein and mRNA level, and of TAP-1 and TAP-2 mRNA. Perhaps more importantly, IFN-γ transfected cells were recognized by the MAGE-3-specific, A2-restricted antimelanoma CTL clone 297/22, while unmodified cells or cell transfected with the IL-2 gene alone were not. These data indicate that IFN-γ gene transfection into HLA-deficient SCLC cells is able to restore their ability to present endogenous tumor antigens to CTL and that IFN-γ gene transfer approaches may be attempted to induce specific CTL responses in SCLC.