Identification of trophoblast in chorionic villi biopsy samples

Angela E. Covone; P. M. Johnson

doi:10.1007/BF00283940

Identification of trophoblast in chorionic villi biopsy samples

Angela E. Covone, P. M. Johnson

Istituto "Giannina Gaslini"

Research output: Contribution to journal › Article › peer-review

Abstract

We have investigated a test for rapid discrimination between foetal and maternal origin of chorionic villi biopsy samples. A monoclonal antibody named H315 reacting against a specific antigen present on the surface of foetal trophoblastic cells, plus a double-colour staining technique (FITC + PI), have been used for the identification of foetal cells (H315-positive) and for visualization of nucleate (PI-positive) and anucleate (PI-negative) structures of chorionic villi. This test could be useful in differentiating foetal and maternal cells in chorionic villi biopsy samples currently used for prenatal diagnostic purposes.

Original language	English
Pages (from-to)	172-173
Number of pages	2
Journal	Human Genetics
Volume	72
Issue number	2
DOIs	https://doi.org/10.1007/BF00283940
Publication status	Published - Feb 1986

ASJC Scopus subject areas

Genetics(clinical)
Genetics

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AB - We have investigated a test for rapid discrimination between foetal and maternal origin of chorionic villi biopsy samples. A monoclonal antibody named H315 reacting against a specific antigen present on the surface of foetal trophoblastic cells, plus a double-colour staining technique (FITC + PI), have been used for the identification of foetal cells (H315-positive) and for visualization of nucleate (PI-positive) and anucleate (PI-negative) structures of chorionic villi. This test could be useful in differentiating foetal and maternal cells in chorionic villi biopsy samples currently used for prenatal diagnostic purposes.

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Identification of trophoblast in chorionic villi biopsy samples

Abstract

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