Identification and purification of overlapping cosmid clones of the region Xq24-qter of human X chromosome using HPLC

Biunno Ida; Mauri Pierluigi; Susani Lucia; Pietta Piergiorgio

doi:10.1016/0888-7543(92)90068-4

Identification and purification of overlapping cosmid clones of the region Xq24-qter of human X chromosome using HPLC

Biunno Ida, Mauri Pierluigi, Susani Lucia, Pietta Piergiorgio

Research output: Contribution to journal › Article › peer-review

Abstract

The assembly of a large physical map of genomes requires simultaneous analysis of many cosmid clones for overlapping regions. The search for overlapping regions may be achieved by various means. High-performance liquid chromatography (HPLC) provides an alternative to gel electrophoresis since microgram amounts of each DNA fragment may be collected into individual test tubes for further analysis. HPLC has been used to identify overlapping cosmid clones from a pool of cosmid DNA containing the terminal portion of the long arm of the human X chromosome (Xq24-qter). Among 400 cosmids analyzed, 3 were shown to overlap.

Original language	English
Pages (from-to)	1353-1355
Number of pages	3
Journal	Genomics
Volume	13
Issue number	4
DOIs	https://doi.org/10.1016/0888-7543(92)90068-4
Publication status	Published - 1992

ASJC Scopus subject areas

Genetics

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10.1016/0888-7543(92)90068-4

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abstract = "The assembly of a large physical map of genomes requires simultaneous analysis of many cosmid clones for overlapping regions. The search for overlapping regions may be achieved by various means. High-performance liquid chromatography (HPLC) provides an alternative to gel electrophoresis since microgram amounts of each DNA fragment may be collected into individual test tubes for further analysis. HPLC has been used to identify overlapping cosmid clones from a pool of cosmid DNA containing the terminal portion of the long arm of the human X chromosome (Xq24-qter). Among 400 cosmids analyzed, 3 were shown to overlap.",

author = "Biunno Ida and Mauri Pierluigi and Susani Lucia and Pietta Piergiorgio",

year = "1992",

doi = "10.1016/0888-7543(92)90068-4",

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T1 - Identification and purification of overlapping cosmid clones of the region Xq24-qter of human X chromosome using HPLC

AU - Ida, Biunno

AU - Pierluigi, Mauri

AU - Lucia, Susani

AU - Piergiorgio, Pietta

PY - 1992

Y1 - 1992

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AB - The assembly of a large physical map of genomes requires simultaneous analysis of many cosmid clones for overlapping regions. The search for overlapping regions may be achieved by various means. High-performance liquid chromatography (HPLC) provides an alternative to gel electrophoresis since microgram amounts of each DNA fragment may be collected into individual test tubes for further analysis. HPLC has been used to identify overlapping cosmid clones from a pool of cosmid DNA containing the terminal portion of the long arm of the human X chromosome (Xq24-qter). Among 400 cosmids analyzed, 3 were shown to overlap.

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Identification and purification of overlapping cosmid clones of the region Xq24-qter of human X chromosome using HPLC

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