Identification and localization of polycystin, the PKD1 gene product

Lin Geng, Yoav Segal, Bernard Peissel, Nanhua Deng, York Pei, Frank Carone, Helmut G. Rennke, Alexandra M. Glücksmann-Kuis, Michael C. Schneider, Maria Ericsson, Stephen T. Reeders, Jing Zhou

Research output: Contribution to journalArticlepeer-review


Polycystin, the product of autosomal dominant polycystic kidney disease (ADPKD) 1 gene (PKD1) is the cardinal member of a novel class of proteins. As a first step towards elucidating the function of polycystin and the pathogenesis of ADPKD, three types of information were collected in the current study: the subcellular localization of polycystin, the spatial and temporal distribution of the protein within normal tissues and the effects of ADPKD mutations on the pattern of expression in affected tissues. Antisera directed against a synthetic peptide and two recombinant proteins of different domains of polycystin revealed the presence of an 400-kD protein (polycystin) in the membrane fractions of normal fetal, adult, and ADPKD kidneys. Immunohistological studies localized polycystin to renal tubular epithelia, hepatic bile ductules, and pancreatic ducts, all sites of cystic changes in ADPKD, as well as in tissues such as skin that are not known to be affected in ADPKD. By electron microscopy, polycystin was predominantly associated with plasma membranes. Polycystin was significantly less abundant in adult than in fetal epithelia. In contrast, polycystin was overexpressed in most, but not all, cysts in ADPKD kidneys.

Original languageEnglish
Pages (from-to)2674-2682
Number of pages9
JournalJournal of Clinical Investigation
Issue number12
Publication statusPublished - Dec 15 1996


  • antibodies
  • cystic disease
  • electron microscope
  • kidney development
  • subcellular localization

ASJC Scopus subject areas

  • Medicine(all)


Dive into the research topics of 'Identification and localization of polycystin, the PKD1 gene product'. Together they form a unique fingerprint.

Cite this