HSV-TK gene transfer into donor lymphocytes for control of allogeneic graft-versus-leukemia

Chiara Bonini, Giuliana Ferrari, Simona Verzeletti, Paolo Servida, Elisabetta Zappone, Luciano Ruggieri, Maurilio Ponzoni, Silvano Rossini, Fulvio Mavilio, Catia Traversari, Claudio Bordignon

Research output: Contribution to journalArticlepeer-review


In allogeneic bone marrow transplantation (allo-BMT), donor lymphocytes play a central therapeutic role in both graft-versus-leukemia (GvL) and immune reconstitution. However, their use is limited by the risk of severe graft-versus-host disease (GvHD). Eight patients who relapsed or developed Epstein-Barr virus-induced lymphoma after T cell-depleted BMT were then treated with donor lymphocytes transduced. With the herpes simplex virus thymidine kinase (HSV-TK) suicide gene. The transduced lymphocytes survived for up to 12 months, resulting in antitumor activity in five patients. Three patients developed GvHD, which could be effectively controlled by ganciclovir-induced elimination of the transduced cells. These data show that genetic manipulation of donor lymphocytes may increase the efficacy and safety of allo-BMT and expand its application to a larger number of patients.

Original languageEnglish
Pages (from-to)1719-1724
Number of pages6
Issue number5319
Publication statusPublished - Jun 13 1997

ASJC Scopus subject areas

  • General


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