HPLC does not differentiate Mycobacterium paratuberculosis from Mycobacterium avium

R. Dei; E. Tortoli; A. Bartoloni; M. T. Simonetti; E. Lillini

doi:10.1016/S0378-1135(98)00292-2

HPLC does not differentiate Mycobacterium paratuberculosis from Mycobacterium avium

R. Dei, E. Tortoli, A. Bartoloni, M. T. Simonetti, E. Lillini

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

HPLC, which is gaining its place as identification tool in mycobacteriology laboratories, has been proposed to distinguish Mycobacterium paratuberculosis from Mycobacterium avium. We had reported no significant difference between M. avium and M. paratuberculosis reference strain ATCC 19698. Because of the advantages offered by such a method, we enlarged our observations to include more isolates of M. paratuberculosis. Within the double cluster of peaks obtained by both M. avium and M. paratuberculosis, we could not find a consistent difference typical of M. paratuberculosis. Therefore, the present study confirmed that M. avium and M. paratuberculosis could not be distinguished by HPLC, raising doubts of a straightforward use of HPLC to identify M. paratuberculosis. Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	209-213
Number of pages	5
Journal	Veterinary Microbiology
Volume	65
Issue number	3
DOIs	https://doi.org/10.1016/S0378-1135(98)00292-2
Publication status	Published - Mar 12 1999

Keywords

Diagnosis-bacteria
HPLC
Mycobacterium paratuberculosis

ASJC Scopus subject areas

Animal Science and Zoology
Microbiology
veterinary(all)

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10.1016/S0378-1135(98)00292-2

Cite this

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abstract = "HPLC, which is gaining its place as identification tool in mycobacteriology laboratories, has been proposed to distinguish Mycobacterium paratuberculosis from Mycobacterium avium. We had reported no significant difference between M. avium and M. paratuberculosis reference strain ATCC 19698. Because of the advantages offered by such a method, we enlarged our observations to include more isolates of M. paratuberculosis. Within the double cluster of peaks obtained by both M. avium and M. paratuberculosis, we could not find a consistent difference typical of M. paratuberculosis. Therefore, the present study confirmed that M. avium and M. paratuberculosis could not be distinguished by HPLC, raising doubts of a straightforward use of HPLC to identify M. paratuberculosis. Copyright (C) 1999 Elsevier Science B.V.",

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T1 - HPLC does not differentiate Mycobacterium paratuberculosis from Mycobacterium avium

AU - Dei, R.

AU - Tortoli, E.

AU - Bartoloni, A.

AU - Simonetti, M. T.

AU - Lillini, E.

PY - 1999/3/12

Y1 - 1999/3/12

N2 - HPLC, which is gaining its place as identification tool in mycobacteriology laboratories, has been proposed to distinguish Mycobacterium paratuberculosis from Mycobacterium avium. We had reported no significant difference between M. avium and M. paratuberculosis reference strain ATCC 19698. Because of the advantages offered by such a method, we enlarged our observations to include more isolates of M. paratuberculosis. Within the double cluster of peaks obtained by both M. avium and M. paratuberculosis, we could not find a consistent difference typical of M. paratuberculosis. Therefore, the present study confirmed that M. avium and M. paratuberculosis could not be distinguished by HPLC, raising doubts of a straightforward use of HPLC to identify M. paratuberculosis. Copyright (C) 1999 Elsevier Science B.V.

AB - HPLC, which is gaining its place as identification tool in mycobacteriology laboratories, has been proposed to distinguish Mycobacterium paratuberculosis from Mycobacterium avium. We had reported no significant difference between M. avium and M. paratuberculosis reference strain ATCC 19698. Because of the advantages offered by such a method, we enlarged our observations to include more isolates of M. paratuberculosis. Within the double cluster of peaks obtained by both M. avium and M. paratuberculosis, we could not find a consistent difference typical of M. paratuberculosis. Therefore, the present study confirmed that M. avium and M. paratuberculosis could not be distinguished by HPLC, raising doubts of a straightforward use of HPLC to identify M. paratuberculosis. Copyright (C) 1999 Elsevier Science B.V.

KW - Diagnosis-bacteria

KW - HPLC

KW - Mycobacterium paratuberculosis

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HPLC does not differentiate Mycobacterium paratuberculosis from Mycobacterium avium

Abstract

Keywords

ASJC Scopus subject areas

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