TY - JOUR
T1 - HIV shedding in cervico-vaginal secretions in pregnant women
AU - Gardella, Barbara
AU - Roccio, Marianna
AU - Maccabruni, Anna
AU - Mariani, Bianca
AU - Panzeri, Lucia
AU - Zara, Francesca
AU - Spinillo, Arsenio
PY - 2011
Y1 - 2011
N2 - Objective: The purpose of this study was to evaluate the presence of HIV-1 in cervico-vaginal secretions of pregnant as compared to non-pregnant HIV-seropositive women. Patients and Methods: We compared 43 known HIV seropositive pregnant patients versus 241 age-matched (± 2 years) control non-pregnant HIV-seropositive subjects. In pregnant patients blood and cervico-vaginal samples were obtained during each trimester of pregnancy. In control subjects the same samples were obtained at enrolment. HIV-1 RNA was measured in plasma; proviral HIV-1 DNA, cell-associated and cell-free HIV-1 RNA in cervico-vaginal secretion by competitive polymerase chain reaction (cRT-PCR) and reverse transcriptase PCR. Results: The genital shedding of HIV-DNA (22/43 as compared to 79/241, p = 0.02), and cell-free HIV-RNA detection (26/43 as compared to 72/241, p
AB - Objective: The purpose of this study was to evaluate the presence of HIV-1 in cervico-vaginal secretions of pregnant as compared to non-pregnant HIV-seropositive women. Patients and Methods: We compared 43 known HIV seropositive pregnant patients versus 241 age-matched (± 2 years) control non-pregnant HIV-seropositive subjects. In pregnant patients blood and cervico-vaginal samples were obtained during each trimester of pregnancy. In control subjects the same samples were obtained at enrolment. HIV-1 RNA was measured in plasma; proviral HIV-1 DNA, cell-associated and cell-free HIV-1 RNA in cervico-vaginal secretion by competitive polymerase chain reaction (cRT-PCR) and reverse transcriptase PCR. Results: The genital shedding of HIV-DNA (22/43 as compared to 79/241, p = 0.02), and cell-free HIV-RNA detection (26/43 as compared to 72/241, p
KW - Cervico-vaginal secretions
KW - HIV shedding
KW - Nucleic acids
KW - Vertical transmission
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U2 - 10.2174/157016211797636017
DO - 10.2174/157016211797636017
M3 - Article
C2 - 21916839
AN - SCOPUS:80053941674
SN - 1570-162X
VL - 9
SP - 313
EP - 320
JO - Current HIV Research
JF - Current HIV Research
IS - 5
ER -