TY - JOUR
T1 - High-performance liquid chromatographic method with fluorescence detection for the determination of total homocyst(e)ine in plasma
AU - Fermo, I.
AU - Arcelloni, C.
AU - De Vecchi, E.
AU - Vigano', S.
AU - Paroni, R.
PY - 1992/2/28
Y1 - 1992/2/28
N2 - A high-performance liquid chromatographic method for the determination of total plasma homocyst(e)ine [H(e)]after reduction with sodium tetrahydroborate and precolumn derivatization with o-phthaldialdehyde is described. The analyses, carried out on a reversed-phase C18 column, were based on spectrofluorimetric detection. The sensitivity was 1 pmol per injection and the intra- and inter-assay relative standard deviations were 1.8% and 5%, respectively. The plasma H(e) concentration determined in 40 healthy volunteers (20-60 years old) was 12.4±2.9 μM (mean±S.D.), in good agreement with reference values.
AB - A high-performance liquid chromatographic method for the determination of total plasma homocyst(e)ine [H(e)]after reduction with sodium tetrahydroborate and precolumn derivatization with o-phthaldialdehyde is described. The analyses, carried out on a reversed-phase C18 column, were based on spectrofluorimetric detection. The sensitivity was 1 pmol per injection and the intra- and inter-assay relative standard deviations were 1.8% and 5%, respectively. The plasma H(e) concentration determined in 40 healthy volunteers (20-60 years old) was 12.4±2.9 μM (mean±S.D.), in good agreement with reference values.
UR - http://www.scopus.com/inward/record.url?scp=0026533946&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026533946&partnerID=8YFLogxK
U2 - 10.1016/0021-9673(92)80283-Z
DO - 10.1016/0021-9673(92)80283-Z
M3 - Article
C2 - 1639902
AN - SCOPUS:0026533946
SN - 0021-9673
VL - 593
SP - 171
EP - 176
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -