Abstract
A new method is described for the determination of submicromolar concentrations of 5-bromodeoxyuridine in human plasma. Sample pretreatment involves cold methanol deproteinization, freezing-thawing and lyophilization. The sample is then analysed by reversed-phase high-performance liquid chromatography. This method is very reproducible and has a detection limit of 0.1 μg/ml (0.32·10-6 M). Comparison with other procedures indicates that the method is advantageous as regards sensitivity and specificity and can be readily applied in clinical pharmacological investigations.
| Original language | English |
|---|---|
| Pages (from-to) | 129-138 |
| Number of pages | 10 |
| Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
| Volume | 491 |
| Issue number | C |
| DOIs | |
| Publication status | Published - 1989 |
ASJC Scopus subject areas
- Chemistry(all)
- Clinical Biochemistry
- Molecular Medicine