High-efficiency antibody discovery achieved with multiplexed microscopy

Shelley Mettler Izquierdo; Stephanie Varela; Minha Park; Ellen J. Collarini; Daniel Lu; Shreya Pramanick; Joseph Rucker; Lucia Lopalco; Rob Etches; William Harriman

doi:10.1093/jmicro/dfw014

High-efficiency antibody discovery achieved with multiplexed microscopy

Shelley Mettler Izquierdo, Stephanie Varela, Minha Park, Ellen J. Collarini, Daniel Lu, Shreya Pramanick, Joseph Rucker, Lucia Lopalco, Rob Etches, William Harriman

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

The analysis of secreted antibody from large and diverse populations of B cells in parallel at the clonal level can reveal desirable antibodies for diagnostic or therapeutic applications. By immobilizing B cells in microdroplets with particulate reporters, decoding and isolating them in a microscopy environment, we have recovered panels of antibodies with rare attributes to therapeutically relevant targets. The ability to screen up to 100 million cells in a single experiment can be fully leveraged by accessing primary B-cell populations from evolutionarily divergent species such as chickens.

Original language	English
Pages (from-to)	341-352
Number of pages	12
Journal	Microscopy (Oxford, England)
Volume	65
Issue number	4
DOIs	https://doi.org/10.1093/jmicro/dfw014
Publication status	Published - Aug 1 2016

Keywords

Antibody discovery
Chicken monoclonal antibody
DR4 DR5
GPCR
Ion channel
Single b cell cloning

ASJC Scopus subject areas

Structural Biology
Medicine(all)
Instrumentation
Radiology Nuclear Medicine and imaging

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10.1093/jmicro/dfw014

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AU - Izquierdo, Shelley Mettler

AU - Varela, Stephanie

AU - Park, Minha

AU - Collarini, Ellen J.

AU - Lu, Daniel

AU - Pramanick, Shreya

AU - Rucker, Joseph

AU - Lopalco, Lucia

AU - Etches, Rob

AU - Harriman, William

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AB - The analysis of secreted antibody from large and diverse populations of B cells in parallel at the clonal level can reveal desirable antibodies for diagnostic or therapeutic applications. By immobilizing B cells in microdroplets with particulate reporters, decoding and isolating them in a microscopy environment, we have recovered panels of antibodies with rare attributes to therapeutically relevant targets. The ability to screen up to 100 million cells in a single experiment can be fully leveraged by accessing primary B-cell populations from evolutionarily divergent species such as chickens.

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KW - Chicken monoclonal antibody

KW - DR4 DR5

KW - GPCR

KW - Ion channel

KW - Single b cell cloning

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High-efficiency antibody discovery achieved with multiplexed microscopy

Abstract

Keywords

ASJC Scopus subject areas

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